水溶性β-NaY(Gd)F_4∶Yb^(3+)/Er^(3+)纳米棒的水热合成及生物偶联

Hydrothermal Synthesis and Bioconjugation of Water-soluble β-NaY(Gd)F_4∶Yb^(3+)/Er^(3+) Nanorods

以聚乙烯亚胺(PEI)为表面活性剂,采用水热合成法,制得了表面氨基修饰的水溶性β-NaY(Gd)F4∶Yb3+/Er3+纳米棒,并对β-NaY(Gd)F4∶Yb3+/Er3+上转换纳米棒的制备方法、条件等进行了考察。结果表明,当Gd3+的引入摩尔分数为40%时,200℃下反应8 h即可获得纯β-NaY(Gd)F4∶Yb3+/Er3+纳米棒。利用X射线粉末衍射仪(XRD)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、荧光光谱(PL)对样品的结构、形貌及光谱特性进行了表征。结构和形貌分析结果表明,制得的样品为单相β-NaY(Gd)F4∶Yb3+/Er3+纳米棒,纳米棒的截面粒径约为40 nm,平均长度约为210 nm。在980 nm波长激发下,样品的上转换发光光谱中出现了4个发射中心位于407,529,546,660 nm的发射带,分别对应于Er3+离子的2H9/2→4I15/2、2H11/2→4I15/2、4S3/2→4I15/2和4F9/2→4I15/2跃迁。采用戊二醛法,使β-NaY(Gd)F4∶Yb3+/Er3+上转换纳米棒表面的氨基与牛血清蛋白(BSA)分子中的氨基成功偶连在一起。利用紫外光谱分析(UV)和考马斯亮蓝法,对二者的偶联进行了证明。

The amino modified β-NaY(Gd) F4∶ Yb3 +/Er3 +nanorods were synthesized by hydrothermal synthesis process using polyethyleneimine(PEI) as a surfactant. The preparation method and conditions of β-NaY(Gd) F4∶ Yb3 +/Er3 +upconversion nanorods were also studied. The pure β-NaY(Gd) F4∶ Yb3 +/Er3 +upconversion nanorods can be obtained at 200 ℃ for 8 h with Gd3 +mole fraction of 40%. The crystal structure,morphology and luminescent properties of final products were characterized by X-ray diffraction(XRD),field emission scanning electron microscopy(SEM),transmission electron microscopy(TEM) and fluorescence spectra(PL),respectively. The assynthesized samples presented a pure phase β-NaY(Gd) F4∶ Yb3 +/Er3 +nanorods with average diameter of 40 nm and average length of 210 nm. Under 980 nm excitation,four upconversion emissionscentered at 407,529,546,660 nm were observed,corresponding to2H9 /2→4I15 /2,2H11 /2→4I15 /2,4S3 /2→4I15 /2and4F9 /2→4I15 /2transitions of Er3 +,respectively. The amino groups on the surface ofβ-NaY(Gd) F4∶ Yb3 +/Er3 +upconversion nanorods can be coupled with bovine serum albumin(BSA) via a glutaraldehyde method,and the coupling was confirmed by UV spectroscopy and Coomassie brilliant blue method at room temperature.