人脐血造血干/祖细胞能在鼠肝中生长

Human Umbilical Cord Blood Hematopoietic Stem/Progenitor Cells Can Grow up in the Mouse Liver

肝脏干 /祖细胞的分化、再生以及它的生物特性是一个非常活跃的研究领域。本研究报告当人脐血细胞经静脉输注给肝脏受损伤的及免疫功能缺陷的小鼠后 ,采用流式细胞术和组织配型 (HLA)的方法 ,结果发现 ,在输注 9周后鼠肝脏细胞内还存在有人脐血细胞 ,即使在灌洗后肝脏细胞的贴壁细胞培养及细胞集落 (CFU GEMM )中 ,也可以发现人脐血细胞的存在。结论 :证实了脐血造血干 /祖细胞能长期在肝脏中生长。

The biological characterization, differentiation and regeneration of hepatic stem/progenitor cells are the one of very active and interested fields. In this report, intravenous injection of human umbilical cord blood(HUCB) cells into the BALB/c nu and SCID mice, an animal model for transplantation and liver injury, was reported. Using of flow cytometry and tissue typing(HLA), it was found that the HUCB cells were survived in mouse liver for 9 weeks. After separation from perfused liver, HUCB cells were detected by hematopoietic colonies(CFU GEMM) in hepatocyte culture. It was concluded that the transplanted HUCB hematopoietic stem/progenitor cells can be survived in the liver over a long period of time.