单独采集血小板和浓缩血小板在保存期中的活化情况

Activation of Random-Donor Platelet Concentrates and Platelet Prepared from Random-Donor Apheresis Collections during Storage

研究单独采集血小板 (单采血小板 )和浓缩血小板在保存期中的活化情况。用流式细胞术对这两种血小板的CD62 p和CD41表达量进行测定。结果表明 :在保存 0 ,1 ,3和 5天时 ,单采血小板的CD62 p阳性率和CD41的平均荧光强度分别为 (1 8 91± 6 2 5) % ,(1 9 48± 8 2 7) % ,(2 2 82± 6 0 6) % ,(56 71± 1 1 79) %及 (8 0 9±2 38) % ,(8 1 3± 2 45) % ,(8 44± 2 51 ) % ,(1 9 87± 6 1 3) % ,而浓缩血小板的分别为 (30 65± 1 2 33) % ,(31 46± 1 1 86) % ,(32 51± 1 3 0 5) % ,(63 55± 1 3 2 7) %及 (1 0 33± 4 37) % ,(1 1 0 9± 6 61 ) % ,(1 3 46± 9 69) % ,(2 4 41± 1 0 1 5) %。二项指标均随保存时间推移而上升。对这两种血小板的计数和 pH值测定显示 ,二者均随保存时间推移而下降。在保存 0 - 3天内两种血小板的计数 ,pH值 ,CD62 p和CD41表达量无显著差异。在第 5天血小板计数和pH值出现显著下降 (P <0 0 0 1 ) ;而CD62p和CD41表达量出现显著上升 (P <0 0 0 1 )。结论

The activation of random donor platelet concentrates and platelets prepared from random donor apheresis collections (plateletpheresis) during storage were studied. Percentage of CD62p staining and mean channel fluorescence(MCF) of CD41 of two kinds of platelets during storage on day 0, day 1, day 3 and day 5 were determined by flow cytometry. The results showed that percentages of CD62p staining and MCF of CD41 in plateletpheresis were (18 91±6 25)%, (19 48±8 27)%, (22 82±6 06)%, (56 71±11 79)% and (8 09±2 38)%, (8 13±2 45)%, (8 44±2 51)%, (19 87±6 13)%, while the results of platelet concentrates were (30 65±12 33)%, (31 46±11 86)%, (32 51±13 05)%, (63 55±13 27)% and (10 33±4 37)%, (11 09±6 61)%, (13 46±9 69)%, (24 41±10 15)%, respectively. The platelet count and pH value were also determined. The platelet number, pH value, percentage of CD62p staining and MCF of CD41 had no significant difference within 3 days of platelet storage. The platelet number and pH value decreased significantly(P<0 001), while percentages of CD62p staining and MCF of CD41 increased significantly(P<0 001) on day 5 of storage. It is concluded that the quality of plateletpheresis is better than platelet concentrate.