摘要
目的 :检测初发及复发转移大肠癌患者外周血中多药耐药基因 (MDR 1)的表达 ,探讨其与病理类型及化疗疗效的关系。方法 :应用逆转录多聚酶链反应 (RT -PCR)法对 5 6例初发大肠癌及 4 0例经术后辅助化疗后复发、转移大肠癌患者的外周血进行检测 ,并与其病理类型及化疗疗效作对比研究。结果 :5 6例初发大肠癌患者外周血中MDR 1阳性表达率为2 7 2 % ,与病理类型无显著相关 (P >0 0 5 ) ;但与肠系膜淋巴结转移密切相关 ,有淋巴结转移者阳性表达率显著高于无淋巴结转移者 (P <0 0 5 ) ;4 0例化疗后复发、转移患者外周血MDR 1阳性表达率为 72 5 % ,与初发大肠癌患者的MDR 1阳性表达率相比差异有显著性 (P <0 0 5 ) ,且MDR 1的表达与化疗疗效呈负相关 ,MDR 1阳性表达者化疗疗效明显低于阴性表达者(P <0 0 5 )。结论 :大肠癌存在先天性和获得性多药耐药性 ;检测外周血MDR 1表达情况可以帮助预测化疗疗效 ,对制定临床化疗方案有指导意义。
Objective:To detect the expression of Multidrug resistance gene(MDR 1) in extroperiblood, evaluate its correlation to pathological features (tipe) and efficiency of chemotherapy.Methods:Extroperblood of 56 cases of primary and 40 case of recurrent metastatic adenocarcinoma of colon and rectum treated by adjuvant chemotherapy after surgical resection was detected by RT PCR. Detect its comparably correlation to pathological features (tipe) and efficiency of chemotherapy.Results:The positive rate of expression of MDR 1 gene in 56 cases of primary stage was 27 2%, without significant difference with pathological features ( P >0 05), but with significantly difference with mesentery lymph nodes, Those with lymph nodes metastasis have significantly higher positive rate ( P <0 05). While the positive rate of 40 cases of recurrent metastatsis was 72 5%,significant difference was observed between primary and recurrent metastatic adenocarcinoma of colon and rectum ( P <0 05). At the same time, expression of MDR 1 has negative correlation to efficiency of chemotherapy. Efficiency of those with positive expression of MDR 1 was greatly (significantly) lower than those with negative one ( P <0 05).Conclusion:Adenocarcinoma of colon and rectum has inherent (congenital) and obtaining (procurement) multidrug resistant gene. Detecting expression of MDR 1 may help to know the efficiency of chemotherapy, and will play some role in strategy of clinical chemotherapy.
出处
《临床肿瘤学杂志》
CAS
2002年第5期321-324,共4页
Chinese Clinical Oncology
基金
黑龙江省"九.五"攻关项目 :(编号 :G99L19- 5)
