脐带血树突状细胞对小鼠S-180移植瘤的作用研究

Study of the Effects of Cord Blood Dendritic Cells on the Growth of Implanted Tumor in Mice

目的 :观察脐带血树突状细胞对小鼠S - 180细胞株移植瘤的作用。方法 :采用脐带血来源的单核细胞制备树突状细胞 ,体外经S - 180细胞肿瘤相关抗原 (TAA)致敏后免疫小鼠 ,一周后接种S - 180细胞株。或先制备荷瘤小鼠模型 ,一周后再接种致敏树突状细胞。结果 :脐带血来源的树突状细胞经胃癌细胞系S - 180的TAA致敏后既能明显保护小鼠该移植瘤的发生 ,又能抑制已发生移植瘤的生长。同对照组相比 ,预防组和治疗组的瘤体积明显缩小 ,细胞免疫功能趋于正常 ,生存期延长 (P <0 0 5 )。结论 :脐带血树突状细胞对小鼠S - 180移植瘤的发生有预防作用 ,对荷瘤小鼠移植瘤的生长有抑制作用 。

Objective:To investigate the immune effects in vivo induced by cord blood dendritic cells (DC) pulsed with tumor extract on the growth of implanted tumor in mice.Methods:DCs were derived from cord blood monocytes, then were pulsed by tumor associated antigen (TAA) extracted from human stomach cancer S-180 cell line. Implanted DC pulsed by the TAA to mice to protect them against the challenge of S-180 cells or to inhibit the growths of implanted tumor.Results:The DC from cord blood pulsed with TAA from S-180 tumor cells can both initiate T lymphocyte immune response to inhibit the growth of implanted tumor in mice and protect them against the challenge of S-180 cells ( P <0 05, respectively).Conclusion:As a new concept of anti tumor vaccine, DC pulsed by TAA may play an important role in treating stomach cancer in vivo.