氨氮对草鱼的急性半致死试验

Experimental study on acute half lethal effects of ammonia nitrogen on grass carp

采用常规生物急性毒性试验方法,在pH 8.85、水温23℃条件下,研究氨氮对草鱼仔鱼存活的影响。试验证明:毒性效应与氨氮浓度呈正相关。平均氨氮TAN浓度为0.31、20.94、30.15、40.28、60.70、91.02、169.99、302.72 mg/L,分子氨NH3浓度为0.02、0.12、0.17、0.23、0.36、0.55、1.02、1.82 mg/L时,草鱼仔鱼96 h死亡率分别为0%、0%、10%、10%、25%、45%、60%、100%。氨氮半致死浓度为2.40 mg/L,对应非离子氨浓度为0.62 mg/L,氨氮安全浓度0.24 mg/L,对应非离子氨安全浓度为0.0615 mg/L。

This experiment adopts the conventional biological acute toxicity experiment, studied under the condition of pH 8.85, the water temperature is 23 ℃, ammonia nitrogen effect on the survival of grass carp larvae. Results show that the toxic effect was positively correlated with ammonia nitrogen concentration and poisoning time, grass carp larvae in non-ionic ammonia were 0.02, 0.12,0.17, 0.23, 0.36, 0.55, 1.02, 1.82 mg/L of 96 h, the mortality rate of 0%, 0%, 10%, 10%, 25%, 45%, 60%, 100%, corresponding to the concentration of non-ionic ammonia safe concentration of 0.0615 mg/L.Ammonia nitrogen and half lethal concentration of 2.40 mg/L, the corresponding non-ionic ammonia concentration of 0.62 mg/L, ammonia nitrogen safe concentration of 0.24 mg/L, the corresponding concentration of non-ionic ammonia safe concentration of 0.0615 mg/L.