酶催化聚合去除水中双酚A的研究

Removing Bisphenol A from Water by Enzymatic Catalyzed Polymerization

该文对辣根过氧化物酶（hrp）催化聚合去除水中双酚a（Bpa）进行实验研究,考察[h2o2]∶[Bpa]摩尔比、酶活力和腐殖酸（ha）浓度对Bpa去除的影响。结果表明,提高过氧化氢浓度和酶活力能够提高Bpa去除率,同时降低水中中间产物a的浓度。在[Bpa]10 mg/l、[hrp]0.2 u/ml、[h2o2]∶[Bpa]为1∶1,ph 7和25℃的条件下反应30 min,当水中存在210 mg/l ha情况下,Bpa去除率从62.2%提高至90%以上。推测机理如下:加入适量的ha后,ha与Bpa发生交叉偶联反应,增大Bpa去除率,当ha加入量过大后,ha可能发生自聚合反应,对Bpa与ha交叉偶联反应产生竞争作用。

The catalyzed polymerization removal of bisphenol A(BPA) by horseradish peroxidase(HRP) was studied,effects of mole ratio of [H_2O_2]∶[BPA],enzymatic activity and humic acid(HA) concentration on BPA removal were investigated.The results showed that:the increase of H_2O_2 concentration and enzymatic activity increased the BPA removal efficiency,and at the same time,the intermediate product A was reduced.Under the condition of [BPA]=10 mg/L,[HRP]=0.2 U/mL,[H_2O_2]∶[BPA]=1∶1,pH 7 and 25 ℃,after 30 min reaction,the presence of 2~10 mg/L HA in the reaction system promoted BPA removal efficiency from 62.2% to more than 90%.It was proposed that enzymatic cross-coupling reaction leads to incorporation of BPA in macromolecules of HA when amount of HA added.However,higher concentration of HA present in the reaction system tends to reduce BPA transformation due to HA seemed to couple to itself.