1株耐受Cu2+好氧反硝化菌及其饥饿特性

Cu^(2+) Tolerance and Hunger Characteristics of an Aerobic Denitrifying Bacterium

文章通过物料平衡分析阴沟肠杆菌Enterobacter cloacae strain F2 (E. cloacae F2)对不同结合态氮的去除能力;考察铜离子(Cu^(2+))对其反硝化影响并对比驯化前后菌株的性能;探究菌株对低碳氮比(C/N)含Cu^(2+)实际生活污水处理效果及饥饿效应,为工程应用提供借鉴。结果表明,菌株可以有效去除浓度为0～200 mg/L时的硝态氮(NO_3^--N)、亚硝态氮(NO_2^--N)和氨氮(NH_4^+-N);当Cu^(2+)浓度达到80mg/L时菌株对于NO_3^--N的去除效率仍高于80%;驯化前后菌株除氮性能无明显区别,且可以同时去除部分Cu^(2+)。此外,菌株对低C/N污水的处理效果在外加碳源后明显增强;经历饥饿状态后补充碳源,可以刺激细菌并提高脱氮效果。因此,菌株E. cloacae F2具有良好的异养硝化-好氧反硝化能力,对于高C/N含Cu^(2+)污水可以表现良好的处理效果。

Enterobacter cloacae strain F2(E.cloacae F2)was applied for nitrogen removal ability study on different forms of nitrogen by mass balance analysis.Influence of Cu2+on aerobic denitrification and comparison of the strains before/after domestication were investigated.Actual treatment trial and starvation effect of strain E.cloacae F2 were studied with the actual low C/N ratio copper-containing domestic sewage,and the results might be reference for practice.NO3--N,NO2--N and NH4+-N could be degraded effectively by strain E.cloacae F2 within a certain range of concentration(0~200 mg/L).The removal efficiency of NO3--N could achieve more than 80%even the concentration of Cu2+was 80 mg/L.The denitrification performances of strains did not show significant differences before or after domestication,and some Cu2+was removed at the same time.The strain was stimulated and nitrogen removal efficiency increased obviously by adding external carbon source.Nitrogen removal ability might also increase by adding external carbon source after starvation.The strain E.cloacae F2 could show satisfied ability of heterotrophic nitrification-aerobic denitrification,efficient treatment ability on high C/N ratio copper-containing domestic sewage.