一体化厌氧氨氧化反应器工艺运行优化研究

Optimization of Nitrosated-ANAMMOX Integration Reactor

文章以上流式厌氧氨氧化反应器为基础,通过接种亚硝化污泥并提高溶解氧(DO)来启动一体化厌氧氨氧化反应器。考察了一体化反应器启动稳定过程中脱氮效能的变化,同时通过高通量测序技术对反应器内微生物群落演替进行了研究。结果表明:一体化反应器在总氮负荷0.75 kg/(m^3·d),DO含量2 mg/L的条件下,氨氮去除率和总氮去除率稳定在85%和80%以上。反应器中参与亚硝化反应的是亚硝化单细胞菌属,厌氧氨氧化反应是Candidatus kuenenia和Candidatus brocadia 2个属参与,反应器内还检测到少量Denitratisoma属的反硝化细菌。除此之外还有许多其他种类的细菌存在。

The nitrosated-ANAMMOX integration process was successful started up in the up-flow ANAMMOX reactor by inoculating nitrite sludge and increasing the dissolved oxygen(DO).The change of nitrogen removal efficiency in nitrosatedANAMMOX integration reactor was studied,and the microbial community structure of the reactor was analyzed by highthroughput sequencing.The reactor was operated at 30℃and DO 2 mg/L.During the start-up of reactor,the nitrogen loading rate was 0.75 kg/(m^3·d),the average ammonium removal efficiency and the total nitrogen removal were 85%,80%respectively.The ammonia oxidizing bacteria Nitrosomonas together with the anaerobic ammonia oxidizing bacteria Candidatus brocadia and Candidatus kuenenia were detected as the functional bacteria in reactor.Besides,some other bacteria like Denitratisoma also existed.