大鼠烧伤后库普弗细胞在促炎细胞因子产生中的作用

The role of Kupffer cells on the postburn production of TNFα, IL-1β and IL-6 in severely scalded rats

目的 观察大鼠严重烧伤后早期 ,库普弗细胞在肿瘤坏死因子α(TNFα)、白细胞介素(IL) 1β、IL 6产生中的作用。方法 观察 (1)烧伤血清对体外培养的大鼠库普弗细胞分泌TNFα、IL 1β、IL 6的刺激作用 ;(2 )烧伤后大鼠库普弗细胞的细胞因子mRNA表达变化 ;(3)应用库普弗细胞特异性抑制剂三氯化钆后 ,烧伤大鼠血浆内细胞因子含量变化。 结果 烧伤血清能刺激库普弗细胞释放TNFα、IL 1β、IL 6 ;大鼠烧伤后库普弗细胞TNFα、IL 1β、IL 6mRNA表达量显著升高 ;预先抑制库普弗细胞的活性 ,烧伤后血浆TNFα、IL 1β、IL 6水平均显著降低 ,分别为烧伤组的 34.71%、36 99%、33.70 %。结论 库普弗细胞是大鼠烧伤后血浆中TNFα、IL 1β、IL

Objective To observe the role of Kupffer cells in the postburn production of TNFα, IL 1β and IL 6 in severely scalded rats. Methods (1) The production of TNFα, IL 1β and IL 6 from rat Kupffer cells stimulated by burn serum was observed. (2) The postburn change in the expression of cytokine mRNA from rat Kupffer cells was monitored. (3) The change in the plasma cytokine contents in scalded rats was determined after the application of gadolinium chloride,a specific inhibitor of Kupffer cells. Results Kupffer cells could be stimulated by burn serum to release cytokines TNFα, IL 1β and IL 6. The mRNA expression of TNFα, IL 1β and IL 6 from rat Kupffer cells increased significantly after injury. But the postburn plasma levels of TNFα, IL 1β and IL 6 decreased obviously to 34.71%, 36.99% and 33.7% of those in scalding group, respectively, after the Kupffer cell activity was inhibited. Conclusion The plasma cytokines, i.e. TNFα, IL 1β and IL 6, were primarily produced from Kupffer cells after injury in scalded rats, initiated by TNFα, IL 1β and IL 6 mRNA transcription. [