高效液相色谱法测定龙胆风湿胶囊中吲哚美辛和吡罗昔康的含量

DETERMINATION OF INDOMETACIN AND PIROXICAM IN LONGDAN FENGSHI CAPSULES BY HPLC

目的:建立龙胆风湿胶囊中吲哚美辛和吡罗昔康的含量测定方法。方法:反相高效液相色谱法。选用大连依利特公司的C_(18)分析柱(200mm×4.6mm,10μm),流动相:乙腈-磷酸盐缓冲液(pH=5.0)(40:60),检测波长:254nm,流速:1.0ml/min^(-1)。结果:本法简便、灵敏、准确。线性范围:吲哚美辛、吡罗昔康分别为0.46～4.60μg(r=0.9999),0.39～1.36μg(r=0.9999)。平均回收率分别为 100.3％(RSD=0.68)和101.6％(RSD=1.82)。结论:本文建立的高效液相色谱法,为测定龙胆风胶囊中的吲哚美辛和吡罗昔康的含量提供了可靠的分析方法。

Objective: To establish a HPLC method for determining Indometacin and Piroxicam inLongDan FengShi Capsules. Method: The reversee--phase system consisted of ODS silica gel column (200mm×4. 6mm, 10μm)and phosphate buffer(pH = 5. 0)-acetonitrile (60: 40)as the mobile phase. The cluate with a flow rate of 1. 0ml·min^(-1) was detested at the wavelength of 254nm. Result: The calibration curves of In-dometacin and Piroxicam were respectively within the range of 0. 46～4. 6μg (r = 0. 9999) and 0. 39～1. 36μg (r = 0. 9999), the average recovery were respectively 100. 3% (RSD = 0. 68) and 101. 6% (RSD =1. 82). Conclusion: This method is simple, rapid, accurate and is suitable for the determination of In-dometacin and Piroxicam in Longdan Fengshi Capsules.