摘要
目的:鉴于用紫外分光光度法测定番泻叶中的番泻苷总含量,操作流程长,操作较为复杂、繁琐,操作条件要求苛刻、因此建立用 HPLC法测定番泻苷 A、番泻苷B的方法。用 ODS色谱柱,检测波长 340nm,流动相为乙腈-1mol/L醋酸-醋酸钠缓冲液(pH5.0)(1→10)(35:65)该混合液 1000ml中,加入四庚基溴化铵 2.45g,外标法。结果:加样回收率:番泻苷 A 98.72％RSD=1.62％,番泻苷 B 100.15％ RSD=1.49％。线性范围:SA 0.0464～0.6960μg r_A=0.9999,SB0.0973～1.4600μg r_B=0.9999。该方法操作简便,可靠,易行。
Objective: HPLC method is established for quantitatively determining sennoside A andsennoside B in senna leaf,because determining total sennoside by UV is very. complex and not easier to bedone for the operation. Method: A reversed--phase chromatographic system was used. consisting of anODS silica gel column wlth a mixture of diluted 1 mol/1 pH5. 0 acetic acid--sodium acetate buffer solution(1 in 10)and acetonitrile (17: 8)containing 5 m mol/l tetra--n--heptylammonium bromide as the mobilephase. Detection wavetength is 340nm. Result: The linear range is SA 0. 0464～0. 6960μg,sennoside A is98. 72±1. 12% (n = 6); SB 0. 0973～1. 4600μg, sennoside B is l00. 15±1. 10% (n = 6 ). Conclusion: Themethod is dependable,accurate and easy.
出处
《中国药品标准》
CAS
2002年第5期48-50,共3页
Drug Standards of China
