非小细胞肺癌组织中端粒酶逆转录酶表达的临床研究

Expression of Telomerase Reverse Transcriptase(TRT)in Human Non-Small Cell Lung Cancer

目的:探讨端粒酶逆转录酶在肺癌中表达及其临床病理意义。方法:采用兔抗人端粒酶逆转录酶抗体(Telomerasereversetranscriptase,TRT),SP免疫组化方法检测32例经福尔马林固定、石蜡包埋的原发性肺癌及10例正常肺组织和肺癌低转移细胞株95-C、高转移细胞株95-D中端粒酶逆转录酶的表达状况。结果:肺癌组织中端粒酶逆转录酶表达的阳性率为100%(32/32),TRT表达定位于胞浆和(或)细胞核,其中高表达率为65.6%(21/32),低表达率为34.4%(11/32)。10例正常肺组织TRT表达阴性。癌及癌旁呈反应性增生的细支气管粘膜上皮和肺泡上皮细胞以及间质淋巴细胞、浆细胞、组织细胞等均呈中～强度TRT阳性表达。低转移及高转移肺癌细胞株分别呈TRT低和高表达。TRT高表达与肺癌转移明显相关(P<0.01);但与肺癌病理组织学分型、分级均无明显关系(P>0.05)。结论:TRT表达可能与肺癌的侵袭转移活性相关。TRT在癌间质和癌旁呈反应性增生的正常组织及细胞的表达可能与干细胞的增殖分化相关。TRT蛋白的原位检测更为直观,有利于肺癌的临床病理诊断和预后评估。

Objective:To investigate the clinico-pathological significance of telomease re-verse transcriptase(TRT)in primary lung cancer.Methods:TRT expression in32formalin fixed,paraffin-embeded samples of non-small cell lung cancer (NSCLS)and2lung cancer cell lines with low and high metastatic lung cancer and10corresponding non-cancer tissues were detected by antibody against TRT(H-231)with immunohistochemistry.Results:Positive expression of TRT was100%(32/32)in primary NSCLS and2/2in cell lines of low and high metastatic lung can-cer.TRT was localized in cytoplasm and/or nucleus of cancer cells.In primary lung cancer,strong expression of TRT in LN(+)group was88.9%(16/18),whereas in LN(-)group was35.7%(5/14)and in normal lung tissue group was0%(0/10)(P<0.01).The expression of TRT is not corre-sponding to the histological classification and the cell differentiation of NSCLC.Expression of TRT could also be found in normal reactive non-tumoral cells in the tumoral and the adjacent tissues,such as proliferative bronchial and alveolar epithelial cells,lymphatic cells,plasma cells and macrophages.Conclusion:TRT expression is closely correlated with lymph node metastasis in NSCLC.Thus it might be one of the new markers for predicting prognosis,reflecting the tendency in lymph node metastasis.TRT expression in non-tumoral tissues might reflect the proliferation and differentiation of stem cells.