上海明代古尸肌肉脂类成分分析

Lipid Analysis of the Muscle From an Ancient Corpse Found in Shanghai

目的 分析测定上海地区出土的明代古尸小腿腓肠肌的脂类成分。上海作为一个现代城市 ,少有古代尸体发现 ,因此对本例古尸脂类成分与其他地区发现的古尸脂类成分进行比较研究。方法 古尸肌肉组织经过预处理以后 ,制成组织匀浆液 ,经冷冻干燥 ,用氯仿 /甲醇抽提和葡聚糖凝胶层析提取分离总脂 ,用三氯化铁法测定胆固醇 ,定磷法测定磷脂 ,甲基橙比色法测定神经氨基醇。结果 测定结果表明该古尸总磷脂含量为 (6 8.14± 2 .2 0 )mg/ g氮 ,胆固醇含量为 (191.6 8± 0 .0 1)mg/g氮 ,神经氨基醇为 (11.49± 3.40 ) μg/mg氮 ,与其他古尸及现代甲醛固定尸体的脂类成分相比 ,总磷脂水平无多大差别 ,而胆固醇及神经氨基醇含量较高。结论 分析结果表明本例古尸保存较好 ,生前可能存在脂类代谢异常。

Purpose: The aim of this paper is to analyze lipids of the gastrocnemial muscle specimens from an ancient corpse that was found in Shanghai which is a modern city. Methods: After removing non-lipid contaminants in the muscle sample, the tissue lysis was used for the extraction of total lipids by means of chloroform/methanol and sephadex chromatography. Cholesterol was determined by ferric chloride method, phospholipids by phosphate measurement, and sphingosine by sephadex gel chromatography and spectrophotometric determination. Results: The results showed that phospholipids content was (68.14 ± 2.20) mg/g of nitrogen in the corpse muscle, cholesterol (191.68 ± 0.01)mg/g N, and sphingosine (11.49 ± 3.4)μg/mg nitrogen. As compared with the results of other area corpses(the ancient corpse from Low-Lan area) and of formalin-fixed corpse, it was found that cholesterol was significantly higher, sphingosine was slightly higher, but the phospholipid level was just similar. Conclusions: Our results suggested that the ancient corpse that we analyzed was well conserved. Maybe the higher contents of cholesterol and sphingosine were associated with the abnormal lipid metabolism before the person died.