补肾泻肝方对辐射诱导小鼠骨髓有核细胞凋亡及基因表达影响

The Effect of BuShenXieGanFang on Apoptosis and Gene Expression of Bone Marrow Nucleated Cells of Mice After Radiation

目的 探索补肾泻肝方 (“BuShenXieGanFang” ,简作补泻方 ,“BXF”)减少辐射所致小鼠骨髓有核细胞(BMNCs)凋亡的作用机制。方法 用TdT介导的脱氧核苷酸切口和末端标记法 (Tunel)检测补泻方对辐射诱导的小鼠BMNCs凋亡的影响 ;应用RT PCR方法检测补泻方对辐射诱导的小鼠BMNCsIL 1和wtp5 3基因表达水平的调控作用。结果 补泻方能明显减少 4Gy照射后 2～ 2 4h的BMNCs凋亡。在 2～ 10Gy剂量照射后 6h ,补泻方能减少BMNCs凋亡 ,尤以 2、4、8、10Gy为显著。辐射诱导IL 1和wtp5 3基因表达 ,基因表达随辐射剂量增加而增加。用药组骨髓细胞经 2、4、6和 8Gy照射后IL 1基因表达显著高于对照组 (P <0 .0 5 ) ;用药组骨髓细胞经 2、4、6和 8Gy照射后wtp5 3基因表达显著低于对照组 (P <0 .0 5 )。显示补泻方能增加辐射所诱导的内源性辐射防护剂IL 1基因的表达 ;降低辐射所诱导的与造血细胞凋亡有关的凋亡基因wtp5 3的表达。 结论 补泻方显著改善辐射所致骨髓细胞凋亡的机制与调控辐射所诱导的与造血细胞凋亡有关的IL 1和wtp5 3基因的表达有关。

Purpose: To understand the mechanisms of decreasing apoptosis of bone marrow nucleated cells (BMNCs) of Mice after radiation by ' BuShenXieGanFang,BXF' treatment. Methods: Apoptosis induced by radiation treatment was detected by TUNEL assay. The levels of IL1 and wtp53 gene expression in the BMNCs of mice after radiation were compared by Reverse transcriptase-polymerase chain reaction (RT-PCR). Results: BXF reduced apoptosis of BMNCs induced by 4 Gy radiation 2-24 h after exposure and 2,4,8 and 10 Gy radiation 6 h after exposure. RT-PCR assays revealed the gene expression of IL-1 and wtp53 induced by radiation with dose-dependent efficiency. The levels of IL1 gene expression of bone marrow cells which be treated by BXF after 2,4,6 and 8 Gy exposure, were significantly increased above the control group, while the levels of wtp53 gene expression were significantly decreased below the control group. Conclusions: These results indicated the mechanisms which decreased apoptosis of BMNCs after radiation by 'BXF' were associated with increasing IL1 gene, decreasing wtp53 gene expression level of BMNCs induced by radiation.