CD226和9.1C3特异性抗体对NK-92细胞杀伤作用的影响

Effect of anti-CD226 mAb and anti-9.1C3 mAb on the cytotoxicity of NK-92 cell line

目的 :探讨NK细胞活化型受体CD2 2 6和NK细胞抑制型受体 9 1C3分子特异性抗体对活化NK细胞系NK 92杀伤的调节作用。方法 :通过间接免疫荧光染色和FCM分析 ,鉴定CD2 2 6和 9 1C3分子在NK 92细胞的表达 ,采用51 Cr释放实验和重导向杀伤实验观察CD2 2 6mAb和 9 1C3mAb对NK 92杀伤作用的影响。结果 :NK 92细胞为CD2 2 6阳性 ,9 1C3弱阳性。CD2 2 6mAb和 9 1C3mAb对NK 92自然杀伤作用没有明显的调节作用。在重导向杀伤实验中 ,CD2 2 6mAb能增强NK 92对P815细胞的杀伤作用 ,而 9 1C3mAb对NK 92的自然杀伤以及CD2 2 6mAb诱导的杀伤均没有抑制作用。结论 :NK细胞活化型受体CD2 2 6分子可能参与NK 92细胞杀伤作用的正向调节 ,而NK细胞抑制受体 9 1C3分子对NK 92细胞杀伤功能的影响不大。

Objective:To investigate the effect of NK cell activating receptor CD226 molecule and NK cell inhibitory receptor 9.1C3 molecule on the cytotoxicity of NK 92,an activated NK cell line.Methods:NK 92 cells were stained with mAbs plus FITC labeled goat anti mouse IgG mAb,and then analyzed by FCM to detect the expression of CD226 and 9.1C3 molecules.The effects of CD226 mAb and 9.1C3 mAb on the cytotoxicity of NK 92 cell were investigated by 51 Cr release experiment and redirected cytotoxicity assay.Results:NK 92 cell line is CD226 positive and 9.1C3 is weak positive.In redirected cytotoxicity assay,CD226 mAb increased the cytotoxicity of NK 92 against P815 cells.However,9.1C3 mAb failed to inhibit both natural cytotoxicity and CD226 mAb mediated cytotoxicity of NK 92 cells.Conclusion:CD226 molecule,a NK cell activating receptor,may be involved in the positive regulation of cytotoxicity of NK 92 cell,whereas NK cell inhibitory receptor 9.1C3 molecule does not affect the cytotoxicity.