环孢素A对稳定EBV诱发淋巴瘤模型的作用和意义

Effect of Cyclosporine A on stable established lymphoma models induced by Epstein-Barr virus

目的 :观察环孢素A(CSA)对huPBL SCID嵌合体小鼠发生移植物和抗宿主反应 (GVHR)的抑制作用 ,建立稳定的EBV诱发淋巴瘤动物模型。方法 :从健康成人新鲜外周血中分离出淋巴细胞 ,将之移植到SCID小鼠腹腔中 ,实验感染EB病毒 ,腹腔注射CSA ,并采用ELISA检测小鼠血清中人sIL 2R水平。结果 :环孢素A组 (14只 )无 1只小鼠因GVHR而死亡 ,而其余 3组共 39只小鼠有 15只因GVHR而死亡 ,中位生存时间为 17d ,死亡率分别为 5 5 5 6 % (5 9例 )、30 4 3% (7 2 3例 )、4 2 86 %(3 7例 ) ,与环孢素A组比较差异有显著性意义。环孢素A组在不同时间sIL 2R含量较稳定 ,而实验感染组sIL 2R水平有逐渐升高趋势 ,环孢素A组与实验感染组同一时间比较 ,15d和 2 2d时sIL 2R水平有显著性差异 ,后者明显高于前者。渡过急性GVHR期而存活的 38只SCID小鼠中 ,共有 2 4只形成肿瘤。结论 :CSA可显著抑制hu PBL SCID嵌合体小鼠GVHR的发生 ,对稳定建立EBV诱发淋巴瘤动物模型具有重要的实际意义。

Objective:To study the effect of Cyclosporine A(CSA) on inhibiting graft versus host reaction(GVHR) occured in hu PBL/SCID chimeras and to stably establish EBV induced lymphoma models.Methods:Human peripheral blood lymphocyts were isolated and were inoculated intraperitoneally into SCID mice.Mice were infected with EBV and injected intraperitoneally with CSA.Human sIL 2R in the serum of hu PBL/SCID chimeras were analyzed by ELISA.Results:No mouse was dead in CSA group,whereas 15 mice of the other three groups died of GVHR.The medium life span of no CSA administration mice was 17 days,and motalities were 55.56%(5/9),30.43%(7/23),42.86%(3/7)respectively.The difference was statistically significant between CSA group and the other groups.The levels of human sIL 2R were stable in CSA group while increased gradually in experimental infertion the groups without CSA.Difference was significant at day 15 and day 22 between the EBV infection group without CSA and with CSA administration.Of 38 survival SCID mice,24 mice developed tumors in their body cavities.Conclusion:CSA can strikingly inhibit GVHR that may occur in hu PBL/SCID mice,that could help practical to stably establish the lymphoma models.