上皮内瘤样病变及宫颈癌组织中Ki67、P16的异常表达

Abnormal expression of Ki67 and P16 in cervical carcinoma and precancerous lesions

目的 研究宫颈癌前病变及癌活检标本中Ki6 7、P16蛋白的异常表达 ,探讨其与宫颈癌变的关系及作为早期癌变生物学标志物的可能性。方法 对正常宫颈黏膜上皮、宫颈上皮内肿瘤性病变 (CIN)及宫颈鳞癌活检组织共 6 2例 ,应用免疫组化技术对宫颈癌变过程中Ki6 7、P16蛋白的异常表达进行研究。结果 宫颈癌Ki6 7阳性表达率 90 .5 %,其表达与组织学分级呈正相关 (P <0 .0 1)。P16在宫颈癌中阳性表达率 5 7.1%,而在正常宫颈黏膜上皮表达率为 90 .4%,其表达与宫颈癌组织学分级有显著差异 (P <0 .0 5 )。结论 Ki6 7、P16蛋白异常表达与宫颈癌显著相关 ,提示将二者联合起来作为筛选癌前病变高危个体的生物指标可能会更有意义。

Objective To investigate alterations in expression of Ki67 and P16 proteins in cervical carcinogenesis.Methods The abnormal expression of Ki67 and P16 proteins was detected by immunohistochemical staining of 62 cases of normal cervical tissue, cervical intraepithelial neoplasia and cervical squamous cell carcinoma.Results The positive rates of Ki67 were 90.5% in cervical carcinoma. A positive correlation was found between the Ki67 expression and the pathological grading of the lesions (P<0.01). The positive rates of P16 were 57.1% in cervical carcinoma and 90.4% in normal cervical tissue.There was a significant difference between the expression of P16 and the cervical carcinoma pathological grading of the lesions(P<0.05).Conclusions There was a positive correlation between the abnormal expression of Ki67 and P16 and cervical carcinoma.It is suggested that Ki67 and P16 expression may be taken as an effective biomarkers for early detection.