一种G蛋白偶联受体-趋化因子受体的表达方法

Methods for expressing chemokine receptors-a G protein-coupled receptor family

趋化因子受体为7次跨膜蛋白G蛋白偶联受体,在人体疾病及肿瘤的发生和发展中扮演重要角色,其结构破解对未来分子靶向药物开发具有巨大的潜在价值。趋化因子受体的成功表达是破解其结构的前提条件。然而,由于跨膜结构的特殊性,其表达方法相较于一般蛋白更具有挑战。通过对趋化因子受体在大肠杆菌、哺乳动物细胞、以及昆虫细胞表达系统内的表达方法进行综述,为相关趋化因子受体的表达方法提供参考。

Chemokine receptors are seven transmembrane protein G-protein coupled receptors,which play important role in human diseases and tumor development.Their structural information has significant and potential value in the development of molecular targeted drugs.Successful expression of chemokine receptors is a key prerequisite for its structure characterization.However,due to membrane structure,the expression method is much more challenging compared with general proteins.This paper reviewed the expression methods of chemokine receptors in E-coli and mammalian cell and insect cell systems providing a method reference for future expression of chemokine receptors.