许旺细胞源神经营养因子在运动神经元轴突逆行运输的研究

Study of retrograde axonal transport of 125 I-Schwann cell derived neurotrophic factor by motor neurons

目的 研究许旺细胞源神经营养因子能否被脊髓运动神经元逆行运输。 方法 氯氨T氧化法标记许旺细胞源神经营养因子 ,新生SD大鼠经一侧小腿肌内注射12 5I标记的许旺细胞源神经营养因子 ,测定左、右侧腰段脊髓的放射性记数。 结果 注射侧腰段脊髓放射性记数明显高于对侧。结论 脊髓运动神经元可以逆行运输许旺细胞源神经营养因子。

Objective To study the retrograde axonal transport of Schwann cell derived neurotrophic factor(SDNF)by motor neurons Methods SDNF was labeled with Chloramine T, 125 I SDNF and 100 fold excess of unlabelled SDNF was injected into the right hindlegs of neonatal rats separately The spinal cords was removed for counting in a  counter Results The radioactive level of experimental side was more than contralateral side The transport was blocked by 100 fold excess of unlabelled SDNF Conclusion SDNF was transported retrogradely by spinal motor neurons