^(125)碘-辣根过氧化物酶评价手术后神经再生的实验

Experimental study of evaluation nerve regeneration with ~ 125 Iodine-horseradish paroxidase after operation

目的 应用放射性同位素追踪手术后神经轴浆流 ,建立评价术后神经再生的实验方法。 方法 SD大鼠 18只 ,将右侧坐骨神经于坐骨结节远侧 0 8cm处切断后立即进行端 端吻合术。 4周后 ,将放射性12 5碘标记的辣根过氧化物酶 (12 5I HRP) 5 0 0 μl注入同侧小腿三头肌。注射后 2 4h ,取吻合口近段神经干 ,应用放射免疫γ 计数器进行组织放射性同位素强度的测定 ;同时取吻合口远段神经干 ,用图像分析系统进行神经干的神经纤维计数。将近段神经组织放射性强度和远段神经纤维计数进行相关分析。 结果 当吻合口近段神经组织中放射性强度增加时 ,吻合口远段神经干神经纤维计数相应增加 ,两者的变化有相关关系 ,差异有显著性 (P <0 0 1)。 结论 手术后神经纤维的再生状况可以用神经组织中放射性同位素强度来表示。放射性同位素追踪手术后神经轴浆流可以作为评价手术后神经再生的一种方法。

Objective To establish a experimental method for evaluating nerve regeneration after operation by means of tracing the retrograde axonal plasm flow by 125 I HRP Methods Eighteen SD rats were used The right sciatic nerve of mouse was transected at the site 0 8cm distal to sciatic notch and then anastomosed immediately Four weeks later,500μl of 125 I HRP solution was injected into the right musculi triceps surae The right sciatic nerve segment proximal to the anastomosis site was cut for checking up the intensity of radioactivity in each tissue at 24 hours after injection The fiber number of nerve distal to the site of anastomosis were calculated The relationship between the intensity of radioactivity and the fiber number was analysed Results The relationship between the intensity of radioactivity of sciatic nerve segment proximal to the site of anastomosis and the fiber number of distal sciatic nerve segment was correlation and there was statistic significance. Conclusion The status of nerve regeneration distal to the site of anastomosis can be evaluated by means of intensity of 125 Iodine in the nerve segment proximal to the site of anastomosis