周围神经缺损神经移植修复对神经元细胞凋亡的影响

Protection effect of nerve implantation after peripheral nerve injury to rats

目的 应用移植神经的方法修复周围神经损伤 ,观察其对神经元的保护作用。 方法 选用雄性SD大鼠 30只。随机分成正常对照组、神经缺损组、神经损伤组、神经移植组 ,实验组每组9只大鼠 ,并按手术先后随机分成 7、1 4、2 8d 3个时间组。将大鼠左侧坐骨神经在梨状肌下缘碾挫或切除 0 5cm ,分别制备神经损伤或缺损动物模型 ,并采用神经原位移植的方法修复神经缺损。按术后不同时间处死动物。于术后 7、1 4、2 8d取L4～ 6 脊髓作TUNEL标记检测 ,标记凋亡的运动神经元数目。切片作HE、甲苯胺兰染色后计算脊髓内运动神经元的数目。 结果 在术后 2 8d ,神经移植组的凋亡细胞明显少于另 2组 (U1 =2 1 0 ;U2 =2 89P <0 0 5)。各时间组的神经元数目 ,神经移植组明显多于另 2组 (t1 =6 84;t2 =6 95P <0 0 5)。 结论 移植神经对周围神经损伤后的相应神经元 ,有一定的保护作用。

Objective To investigate the protection effect of nerve implantation to the neurons after sciatic nerve injury to adult rats. Methods Thirty male Sprague Dawley rats weighing 180-220 g (8-9 weeks of age) were randomly divided into four groups. Group A consisted of normal rats without operation. In group B, the sciatic nerve of rats was transected, with the proximal stump of the sciatic nerve ligated to inhibite nerve regeneration. In group C, a sciatic nerve crushed model was set up. In group D, a sciatic nerve implantation model was established. The rats were sacrificed on postoperative days 7,14 and 28 respectively. The L 4 L 6 segments of the spinal cord were harvested. TUNEL technique was used to detect apoptotic motor neurons. HE and Toluidine Blue staining was used for counting motor neurons. Results The apoptotic neurons detected on the 28th postoperative day were significantly fewer in the implantation group than in the other groups ( P <0 05). The number of motor neurons was significantly higher in the implantation group than in other two control groups ( P <0 05). Conclusions Nerve implantation exerts protective effect on neurons after nerve injury.