固相微萃取-气相色谱法检验盐酸氯丙嗪

Determination of Chlorpromazine Hydrochloric by Solid Phase Microextraction-Gas Chromatography

本文研究了固相微萃取 (SPME) -气相色谱法提取检验盐酸氯丙嗪的最优条件。结果表明 ,以下实验条件效果最好 :以涂敷有 30μm厚的聚二甲基硅氧烷的石英纤维直接萃取水中盐酸氯丙嗪时 ,溶液 p H值为 8,吸附温度为 80℃ ,萃取 2 0 min,在 2 5 0℃下解吸 5 min。在 1— 10 0 0μg/ L浓度下 ,色谱峰面积与溶液浓度呈线性关系 ,其回归方程为 y=16 4 6 5 +180 7.9x (r=0 .99997) ,检出限为 0 .84μg/ L ,用此法测定茶水中盐酸氯丙嗪 ,平均回收率为 96 .0 5 %。

The best operation conditions were studied for the determination of chlorpromazine hydrochloric by solid phase microextraction gas chromatography. A silica fibre coated with a polydimethylsiloxane film of 30μm in thickness was immersed directly into the solution . Chlorpromazine hydrochloric in the solution was then extracted onto the film at 80℃ for 20min at pH 8,and was desorbed at 250℃ for 5min. A linear relationship between the peak area of GC and the sample's concentration is y =16465+1807.9 x (r =0.99997),in the concentration range of 1-1000μg/L, with the detection limit 0.84μg/L. The method has been applied successfully to the analysis of chlorpromazine hydrochloric in tea with the recovery of 96.05%.80 7.9x (r=0 .99997) ,in the