PSA试验在精斑检验中的应用探讨

The Discussion of semen stains examination in the field of forensic applications

目的通过比较、分析PSA实验结果,探讨PSA试验在精斑检验中的合理应用。方法对检材进行PSA试验后,通过差异裂解法获得上清及沉渣STR分型。结果经STR检测,与PSA试验结果存在不一致,其中假阳性13例,假阴性1例;上清中检出男性成分2例,男女混合9例。结论 PSA试验存在一定假阳性及假阴性率,检验经差异裂解法分离得到的上清不仅可以增加精斑检验的成功率,还可以作为补充为精斑检验带来更多有价值的信息。

Objective To investigate the significance of a comprehensive test on the semen stain in the field of forensic evidence; Methods PSA tests performed on samples obtained supernatant and sediment STR genotyping by differential lysis method; Results Through STR testing, there are inconsistencies with the PSA test results in which 13 cases of false positive and false negative in 1 case. The supernatant was detected in two cases of male component, coeducational 9 cases; Conclusion PSA test strips there are some false positive and false negative rate, test isolated by the difference lysis supernatant can not only increase the success rate of the fine on the semen stain can also as a supplement to bring more valuable information for the semen stain.