基于27-plex SNPs的四川地区四个族群遗传推断研究

Genetic inference study of four populations in Sichuan province by 27-plex SNPs

目的基于27-plex SNPs族群推断体系对四川地区汉族、藏族、羌族及彝族四个族群遗传结构及成分进行研究。方法使用27-plex SNPs试剂检验四个族群1054份样本并获取SNPs位点分型,利用主成分分析、聚类分析及系统发育树综合分析族群间遗传关系,使用族群推断软件(DNA Ancestry Analyzer,DAA)推断样本的族群来源并统计推断结果的准确性。结果系统发育树显示四川汉族、藏族、羌族、彝族和其他东亚族群共同聚为一支;主成分分析和聚类分析结果显示四川汉族、藏族、羌族及彝族主要祖先成分均为东亚。除1例样本祖先来源不排除东亚或混合族群外,其余1053份样本均来源于东亚。结论四川地区汉族、藏族、羌族和彝族均为典型的东亚族群,藏族、彝族和羌族的遗传关系较近。使用27-plex SNPs族群推断系统对四川汉族、藏族、羌族和彝族进行遗传推断,结果可靠。

Objective To study the genetic structure of Han,Tibetan,Yi,and Qiang in Sichuan using the 27-plex SNP panel.Methods In total,1054 individuals of four populations from Sichuan province were tested using 27-plex SNPs.Principal component analysis,Structure and phylogenetic tree were used to analyze the genetic relationship.DNA Ancestry Analyzer(DAA)was used to analyze the ancestry components and infer the ancestry of all the test samples.Results The phylogenetic tree showed that Sichuan Han,Tibetan,Yi,Qiang were clustered with other East Asian groups.The STRUCTURE and principal component results validated that the common ancestry source of all samples from the four ethnic groups is East Asian except 1 sample.Conclusions The samples of four ethnic groups were assigned into East Asian,and Tibetan,Yi and Qiang are more genetically close,which is consistent with previous linguistics,history,and biology reports.In conclusion,the ancestry inference results of all the tested individuals from four populations in Sichuan province are reliable.