糖萜素对小鼠腹腔巨噬细胞产生一氧化氮的影响

The effects of saccharicterpenin on the production of NO by mice peritoneal macrophages

目的 :探讨糖萜素对小鼠腹腔巨噬细胞产生NO的影响。方法 :在饲料中添加不同剂量糖萜素喂养NIH小鼠 ,以基础饲料为对照 ,检测环磷酰胺免疫抑制组与非抑制组不同时间段小鼠腹腔巨噬细胞产生NO的含量。结果 :含糖萜素饲料组于不同时间测得NO含量均较基础饲料组显著增高 (P <0 .0 5 )。免疫抑制状态下 ,含糖萜素饲料组NO含量也较基础饲料组高(P <0 .0 5 )。体外糖萜素不能直接影响NO的生成。结论 :糖萜素能显著提高小鼠体内巨噬细胞产生NO。

OBJECTIVE:In order to study the effects of saccharicterpenin on the production of NO by mice peritoneal macrophages. METHOD:NIH mice were fed on the food containing different doses of saccharicterpenin. NO produced by the macrophages from cyclophosphamide immunosuppressive group and non-immunosuppressive group were measured at different times. RESULTS:The levels of NO in mice fed on saccharicterpenin (group B) were significantly different from that in common food group (group A) at different time. After immunosuppression the levels of NO in mice fed on saccharicterpenin were higher than that fed on common food (P<0.05). Saccharicterpenin did not influence the production of NO directly in vitro. CONCLUSION:Saccharicterpenin can activate the mice macrophages to produce NO in vivo, but not influence the production of NO directly in vitro.