三磷酸腺苷二钠氯化钠注射液质量研究

A study on the Quality of Adenosine Disodium Triphosphate and Sodium Chloride Injection

目的 :建立三磷酸腺苷二钠 (ATP)氯化钠注射液中ATP的含量及有关物质测定方法。方法 :采用高效液相色谱法 ,固定相为岛津WAX - 1柱 ,以磷酸氢二钠缓冲液为流动相 ,检测波长为 2 5 9nm ,测定该制剂中ATP及有关物质的含量。结果 :ATP的平均回收率为 10 0 .4% ,RSD %为 0 .32 %。结论 :本法操作简便 ,结果准确 。

OBJECTIVE:To establish a method for the determination of adenosine disodium triphosphate in adenosine disodium triphosphate and sodium chloride injection. METHOD:HPLC was selected to determine the content,column:WAX-1(Shimadzu), detector:uv λ=259nm,mobile phase:0.25mol/L phosphate buffer solution (adjust pH to 7.0).RESULTS:The regression equation was y=9188755 C -7143.4,r=0.9999(10～100mg/ml). The average recovery was 100.43%,(RSD%=0.32%).CONCLUSION:It is a suitable method for the determination of ATP in ATP and NaCl injection.