同型半胱氨酸对大鼠血管钙化模型的影响

Effects of homocysteine on rat model of vascular calcification

目的 探讨同型半胱氨酸 (Hcy)对心血管系统异位钙化的影响。方法 建立大鼠血管钙化模型 (维生素D3加尼古丁 ,VDN) ,将 4 0只大鼠分为 4组 :A组 (对照组 ) ;B组 (VDN组 ) ;C组 (VDN +Hcy组 ) ;D组 (Hcy组 )。 5周后测大鼠体重、尾动脉压后处死 ,测主动脉长度及心脏重量 ,用原子吸收分光光度计测定主动脉钙含量 ,用逆转录聚合酶链反应 (RT PCR)检测 4组大鼠主动脉骨桥蛋白 (OP)mRNA表达。结果 经VDN处理后使大鼠体重减轻 ,而Hcy对大鼠体重无影响 ;去除体重减轻的因素 ,4组大鼠胸腹主动脉长度差异无显著改变 ;VDN及Hcy处理均不影响大鼠心率及尾动脉压 ;VDN大鼠主动脉钙含量较对照组增加 14 .1倍 ,Hcy的加入使其进一步升高达 18.9倍 ,两者相比 ,差异有显著性意义 (P <0 .0 1) ,单纯Hcy处理大鼠的主动脉钙含量无明显变化 ;VDN大鼠主动脉检出OPmRNA表达 ,Hcy促进此表达增加 5 6 .76 % ,对照组大鼠主动脉内无OPmRNA表达 ,单独给予大鼠Hcy处理不能诱发主动脉内OPmRNA表达。结论 Hcy促进VDN大鼠主动脉钙沉积及OPmRNA表达 。

Objective To explore the relation between homocysteine(Hcy) and cardiovascular ectopic calcification in vivo.Methods Rat model of vascular calcification was established by in injection of vitamin D 3 plus intragastric administration of nicotine(VDN).40 rats were divided into 4 groups:control,VDN,VDN+Hcy and Hcy.At the end of 5 weeks,all rats were sacrificed after their body weight and caudal arterial pressure were measured.The aorta was taken,its calcium content was measured by atomic absorption spectrophotometry and the expression of osteopontin (OP) mRNA was sxamined by RT PCR.Results In theVDN groups,in contrast to the absence of change in plasma calcium,treatment with vitamin D 3 plus nicotine produced considerable increase (14.1 times) in aorta calcium content.Hcy aggravated this increase,causing that the aorta calcium content in VDN+Hcy group increased by 18.9 times.However,rats only treated with Hcy did not have this change.OP mRNA was detected in the aorta of VDN rats and Hcy increased the expression of OP mRNA by 56.76%.Conclusions Hcy can increase calcium content and the expression of OP mRNA in aorta of VDN rats,but has no same effect on control rats.It may be a promoting factor for calcification,but not an initiating factor.