摘要
A significant reduction of trypsin inhibitory activity by selected bacterial proteolytic enzymes was demonstrated in vitro.Two trials were conducted to examine the capacity of the tested enzymes to inactivate soybean ANFs in vivo.In trial I,twenty four piglets weaned at four weeks of age were assigned in replicate groups of 4 piglets per pen to one of three dietary treatments:(1)control;(2)Enzyme 1 supplemented(E 1);(3)Enzyme 2 supplemented (E 2).In trial II,twenty piglets weaned at five weeks of age were alloted to five treatment diets:(1)contro,l:(2)0.1% P4 supplemented;(3)0.5% P4 supplemented;(4)0.1% P7 supplemented;(5)0.5% P7 supplemented.The optimum pH for hydrolysis was 8 for E ,9 11 for E 2,8.5 for P4 and nuctral for P7.After 17 days of the trial,daily gain of piglets on enzymes E 1 and E 2 was 36% and 18% more than that in the control group,although the difference was not significant.The animals on the treated groups had a tendency to have lighter heart(7.8 and 5.9%),spleen(11.1 and 7.4%) and pancreas(16.7 and 12.5% for E 1 and E 2 respectively)in relation to empty body weight than those in the control.The small intestine of pigs on the treated groups was significantly lighter(18.9 for E 1 and 7.7% for E 2) than that in the control(P<0.05).The stomach (26.4 and 24%,p=0.198) and cecum(21.9 and 9.4%,p=0.114) also showed the same pattern.The growth depression was attributed to reduced feed intake caused by antinutritional factors in soybeans.It is concluded that supplements of proteolytic enzymes E 1 or E 2 had a positive effect on growth and efficiency and caused much less reaction in the gut as manifested by the weight of the tract and of its accessory organs.Dietary saupplements of P4 or P7 had no significant effect on growth,but reduced reaction of soybean antinutritional factors in the gut,especialy P4 in dose of 0.5%.The growth depression was attributed to low feed intake caused by antinutritional factors in soybeans.
A significant reduction of trypsin inhibitory activity by selected bacterial proteolytic enzymes was demonstrated in vitro.Two trials were conducted to examine the capacity of the tested enzymes to inactivate soybean ANFs in vivo.In trial I,twenty four piglets weaned at four weeks of age were assigned in replicate groups of 4 piglets per pen to one of three dietary treatments:(1)control;(2)Enzyme 1 supplemented(E 1);(3)Enzyme 2 supplemented (E 2).In trial II,twenty piglets weaned at five weeks of age were alloted to five treatment diets:(1)contro,l:(2)0.1% P4 supplemented;(3)0.5% P4 supplemented;(4)0.1% P7 supplemented;(5)0.5% P7 supplemented.The optimum pH for hydrolysis was 8 for E ,9 11 for E 2,8.5 for P4 and nuctral for P7.After 17 days of the trial,daily gain of piglets on enzymes E 1 and E 2 was 36% and 18% more than that in the control group,although the difference was not significant.The animals on the treated groups had a tendency to have lighter heart(7.8 and 5.9%),spleen(11.1 and 7.4%) and pancreas(16.7 and 12.5% for E 1 and E 2 respectively)in relation to empty body weight than those in the control.The small intestine of pigs on the treated groups was significantly lighter(18.9 for E 1 and 7.7% for E 2) than that in the control(P<0.05).The stomach (26.4 and 24%,p=0.198) and cecum(21.9 and 9.4%,p=0.114) also showed the same pattern.The growth depression was attributed to reduced feed intake caused by antinutritional factors in soybeans.It is concluded that supplements of proteolytic enzymes E 1 or E 2 had a positive effect on growth and efficiency and caused much less reaction in the gut as manifested by the weight of the tract and of its accessory organs.Dietary saupplements of P4 or P7 had no significant effect on growth,but reduced reaction of soybean antinutritional factors in the gut,especialy P4 in dose of 0.5%.The growth depression was attributed to low feed intake caused by antinutritional factors in soybeans.
