基于分子印迹聚合物掺杂氧化石墨烯膜的利巴韦林电位传感器

A Ribavirin Potentiometric Sensor Based on Molecularly Imprinted Polymers Doped with Graphene Oxide Film

以利巴韦林为模板分子,甲基丙烯酸为功能单体,采用沉淀聚合法制备利巴韦林分子印迹聚合物(MIP)。以利巴韦林分子印迹聚合物掺杂氧化石墨烯(GO)为离子载体,聚氯乙烯为基质,癸二酸二辛酯为增塑剂制备电极敏感膜。结果表明,敏感膜组成为100.8 mg MIP、14.7 mg GO、450.8 mg聚氯乙烯和901.6 mg癸二酸二辛酯,内充液组成为0.1 mol/L NaCl+0.05 mol/L Na Ac-0.05 mol/L HAc缓冲溶液+1.0×10^(-5)mol/L利巴韦林,电极的响应性能最好。此电极的能斯特响应斜率为45.565 m V/decade,线性范围为1.0×10^(-6)~1.0×10^(-4)mol/L,检出限为1.0×10^(-7)mol/L(S/N=3),工作p H范围为3~5,响应时间小于3 min。此电极对利巴韦林具有高选择性,可用于检测饲料和注射液中利巴韦林含量,加标回收率为90%~110%,RSD为3.0%~7.9%。

Molecularly imprinted polymer( MIP) was synthesized by precipitation polymerization using ribavirin as the template molecule and methacrylic acid as the functional monomer. The sensitive film of ribavirin electrode was constructed by molecularly imprinted polymer doped with graphene oxide as the ionophore,poly( vinylchloride)( PVC) as matrix and dioctyl sebacate for plasticizer. The results showed that the best performance of the electrode was obtained when the sensitive film compositions were 100.8 mg of MIP,14.7 mg of GO,450.8 mg of PVC and 901.6 mg of dioctyl sebacate,and the inner filling solution composition was 0.1 mol/L NaCl + 0.05 mol/L Na Ac-0.05 mol/L HAc + 1.0×10^(-5)mol/L ribavirin solution. The electrode showed a near-Nernstian slope of 45.565 mV/decade for ribavirin over the range of 1.0×10~6-1.0×10^(-4)mol/L and a detection limit of 1.0×10^(-7)mol/L. The electrode could be used in the pH range of 3 to 5 with response time of less than 3 min. The developed electrode exhibited high selectivity for ribavirin and was successfully applied to the determination of ribavirin in feed and injection with recoveries of 90%-110% and RSD of 3.0%-7.9%.