背景光照明对ELISA成像系统的影响

The influence of background lightillumination on ELISA imaging system

提出基于手机比色法的酶联免疫吸附测定(ELISA)检测系统,进行检测波长为450 nm的C反应蛋白(CRP)实验,基于B通道响应的拟合曲线(R2=0. 9996)与酶标仪绘制的标准曲线(R2=0. 9999)具有良好的一致性。在设置的8个未知样本中,浓度检测的最小误差为0. 75%,验证了系统的可行性。研究了ELISA成像系统中的背景光照明模块,分析证明了到达微孔板底部光照的均匀性。最后,对手机采集的不同光通量下的成像图片处理,研究光通量对ELISA实验的影响,光通量在60～100 lm范围内时,R2大且较稳定此时的MSE也最小。一个合适独立的照明模块,有利于成像质量和检测系统精确性的提高,可适用于多种ELISA成像系统。

An enzyme linked immunosorbent assay(ELISA)detection system based on mobile phone colorimetric method was proposed.The detection of C reactive protein(CRP)in 450 nm was carried out.The fitting curve(R2=0.9996)based on the B channel responsewas in good agreement with the standard curve(R2=0.9999)obtained by the laboratory instrument.In the 8 unknown samples,the minimum error of concentration detection was 0.75%,which verified the feasibility of the system.In addition,this paper focused on the background lighting module in ELISA imaging system,and analyzed the uniformity of light at the bottom of the microplate.Finally,the influence of the illumination on the ELISA experiment was studied under the different illuminationconditions.When the luminous flux was within the range of 60-100 lm,the R2 was large and the MSE was small.A suitable and independent lighting module is beneficial to the improvement of imaging quality and accuracy of detection system.It can be applied to various ELISA imaging systems.It is of great significance to the development of realtime diagnosis technology and the development of home self-monitoring technology.