种子蛋白乳酸尿素聚丙烯酰胺凝胶电泳技术及其对向日葵自交系和杂交种的鉴定

Lactate urea polyacrylamide gel electrophoretic technology for seed proteins and identification of sunflower inbreds and hybrids

在对已报道的酸性和碱性两大电泳系统多种方法的比较研究中，我们利用国产仪器和试剂，研究出了一项利用种子蛋白乳酸尿素聚丙酰胺凝胶电泳鉴定向日葵自交系和杂交种纯度的技术。该技术主要包括（１）改进了单粒种子脱脂程序，（２）筛选出有效的向日葵种子蛋白提取剂，（３）确定了凝胶制备的国产丙烯酰胺和亚甲基双丙烯酰胺的新配比，（４）优化了新的酸性凝胶聚合催化系统“Ａｐ－Ｖｃ－ＦｅＳＯ４ 的配比，（５）筛选出新的电极缓冲液，明确了最佳条件。

Based on home made instruments and reagents, an improved lactate urea polyacrylamide gel electrophoretic technology suitable for resolving seed proteins of sunflower varieties was developed. The technology characterized by (1) an alternative procedure of seed defatting, (2) a novel extracting solution of seed proteins, (3) a new ration of Acr and Bis in gel stock solution, (4) new gel polymerization catalyst system ‘Ap—Vc—FeSO 4’ with the best mix ration was employed in gel preparation, (5) new electrode buffer and suitable electrophoretic conditions was determined. The studies showed that the technology may be promising using in sunflower varieties identification.