隔日限食疗法对大鼠急性脊髓损伤后血清TNF-α的影响

Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury

目的:观察隔日限食疗法(EODF)对大鼠急性脊髓损伤后血清肿瘤坏死因子-α(TNF-α)的影响。方法:48只雌性成年Wistar大鼠,随机分成EODF组(A组)、正常饮食对照组(B组)、假手术组(C组);A、B组采用改良Aleln's打击法建立Wistar大鼠胸段脊髓损伤模型,C组仅行椎板切除术而不损伤脊髓;A组于术后立即给予EODF,B组及C组给予正常饮食。3组大鼠分别于术后6 h、1 d、3 d、7 d行动物行为学BBB评分观察大鼠运动功能变化;处死大鼠取血清,运用ELISA法检测各组大鼠血清TNF-α的水平。结果:(1)A、B组大鼠术后均出现严重后肢功能障碍,BBB评分早期(6 h、1 d)差异无统计学意义(P>0.05),术后3 d、7 d2组评分差异有统计学意义(P<0.01);(2)A、B组大鼠术后血清TNF-α含量均出现升高,但2组TNF-α含量在6 h时差异无统计学意义(P>0.05),术后1 d、3 d、7 d 2组含量差异有统计学意义(P<0.05)。结论:EODF能有效改善急性脊髓损伤大鼠后肢运动功能,其作用机制可能与其抑制炎性因子TNF-α产生,发挥抗炎作用,减轻继发性损伤有关。

Objective :To observe the effect of every-other-day fasting(EODF) on serum tumor necrosis factor-α(TNF-α)level in rats after acute spinal cord injury. Methods :A total of 48 female adult Wistar rats were randomly divided into EODF group( group A), normal diet control group(group B) and sham-operated group(group C). Thoracic spinal cord injury models were established in rats of group A and group B by modified Allen contusion method,and laminectomy was performed in rats of group C. Then group A received EODF,group B and group C were given a normal diet immediately after operation. BBB scores were calculated in each group at 6 hours,1st day,3rd day and 7th day after operation to observe the motor function changes,then the rats were killed and their serum was taken. The level of TNF-α in serum was detected by ELISA. Results:Severe hindlimb motor dysfunction of rats was found in group A and group B after operation,and there were significant differences in BBB scores between the two groups at 3rd day and 7th day after operation(P<0.05). The level of serum TNF-α was increased in group A and group B after operation,and there were significant differences in the index between the two groups at 1st day,3rd day and 7th day after operation(P<0.05).Conclusion:EODF can improve the hind limb motor function of rats after acute spinal cord injury. The mechanism may be related with suppressing expression of inflammatory cytokines TNF-α,inhibiting inflammatory response and reducing secondary injury.