电针阳明经穴治疗大鼠失神经肌萎缩疗效研究

Effect of Electroacupuncture at Acupoints of Yangming Meridian on Denervated Muscle Atrophy of Rats

目的:观察电针阳明经穴治疗失神经肌萎缩的临床疗效。方法:60只SD大鼠按照随机数字表法分为对照组、模型组、非经非穴组、他经穴组和阳明经穴组,每组12只。除对照组外,其余4组均行左侧坐骨神经切断术。对照组和模型组不进行电针治疗;非经非穴组、他经穴组、阳明经穴组使用电针仪(疏密波,频率2/33 Hz,强度1.5～2 mA)进行干预。非经非穴组选取足三里穴和上巨虚穴旁开0.5 cm的位置;他经穴组取阴陵泉穴和地机穴;阳明经穴组取足三里穴和上巨虚穴,1次/d,30 min/次,5 d/疗程,共持续治疗4个疗程。治疗4个疗程末,观察坐骨神经功能指数(SFI)、患侧/健侧腓肠肌湿重比(RWW)、患侧/健侧肌纤维横截面积比(RCA)和肌纤维直径比(RFD)、肌细胞凋亡指数(AI),并采用免疫双荧光染色法观察PAX3/PAX7表达情况。结果:与对照组比较,模型组、非经非穴组、他经穴组、阳明经穴组SFI、RWW、RCA和RFD明显降低,AI和PAX7明显升高,差异具有统计学意义(P<0.05);与模型组比较,他经穴组、阳明经穴组SFI、RCA、RFD、PAX7均明显升高,AI明显降低,阳明经穴组RWW、PAX3明显升高,差异具有统计学意义(P<0.05);与他经穴组比较,阳明经穴组RWW、RCA、RFD、PAX3均明显升高,AI明显降低,差异具有统计学意义(P<0.05)。结论:电针阳明经穴可改善大鼠失神经支配肢体功能和组织形态,降低肌细胞凋亡水平,促进肌细胞增殖分化,有效防治肌萎缩。

Objective: To observe the effect of electroacupuncture at Yangming meridian acupoints on denervated muscular atrophy. Methods: A total of 60 SD rats were randomly divided into control group, model group, non-acupoint group, other meridian group and Yangming meridian group, with 12 cases in each group. Except for the control group, the left sciatic nerve were cut off in the other four groups. The control group and model group did not receive electro-acupuncture treatment;non-acupoint group, other meridian group and Yangming meridian group was treated by electro-acupuncture instrument(dense wave, frequency 2/33 Hz, intensity 1.5-2 m A). The non-acupoint group selected Zusanli acupoint and Shangjuxu acupoint 0.5 cm apart;other meridian group took Yinlingquan acupoint and Diji acupoint;Yangming meridian group took Zusanli and Shangjuxu acupoint, Once a day, 30 minutes a time, five days a course of treatment, continuous treatment for four courses. After treatment for four courses, the SFI, RWW, RCA,RFD, AI of affected/contralateral gastrocnemius muscle were observed, and the expression of PAX3/PAX7 was observed by double immunofluorescence staining. Results: Compared with the control group, SFI, RWW, RCA and RFD in the model group, non-acupoint group, other meridian group and Yangming meridian group decreased significantly, AI and PAX7 increased significantly, with statistically significant differences(P<0.05);Compared with the model group, SFI, RCA, RFD and PAX7 in the other meridian group and Yangming meridian group increased significantly, AI decreased significantly, RWW and PAX3 in Yangming meridian group increased significantly, with statistically significant differences(P<0.05);Compared with other meridian group, RWW, RCA, RFD, PAX3 in Yangming meridian group increased significantly, AI decreased significantly, with statistically significant differences(P <0.05).Conclusion: Electroacupuncture at Yangming meridian acupoints can improve the function and histomorphology of denervated limbs,reduce the apoptotic level of muscle cells, promote the proliferation and differentiation of muscle cells, and effectively prevent and treat muscle atrophy of rats.