叶酸对卵巢癌细胞基因组DNA整体甲基化及细胞行为学的影响

The effects of folic acid on OV90 cells global genome DNA methylation and cell behavioral

目的探讨叶酸对卵巢癌细胞OV90基因组DNA整体甲基化及细胞行为学的影响,为卵巢癌的防治提供理论基础。方法体外培养人卵巢癌细胞株OV90,检测不同叶酸浓度作用于癌细胞3个月后对细胞基因组DNA整体甲基化及细胞增殖、细胞周期和细胞凋亡的影响。细胞分组如下:高叶酸组,叶酸浓度为40 mg/L;正常组(常规培养的细胞),叶酸浓度为4 mg/L;低叶酸组,叶酸浓度为0 mg/L。分别采用基因组DNA甲基化检测试剂盒检测基因组DNA的整体甲基化水平;CCK-8检测细胞增殖率;流式细胞术检测细胞周期、细胞凋亡的变化。结果随着叶酸浓度的增加,卵巢癌细胞基因组DNA整体甲基化水平增高;高叶酸组对癌细胞的抑制率显著高于正常组;与正常组相比较,高叶酸组卵巢癌细胞G2/M细胞百分数降低(P<0.05),S期细胞比分数升高(P<0.05),G0/G1期无明显变化(P>0.05);细胞凋亡率呈上升趋势,差异有显著性。结论一定浓度的叶酸可提高卵巢癌细胞OV90基因组DNA的整体甲基化水平,并对其生长存在一定的抑制作用。

Objective To investigate the effects of folicacid on the Global genome DNA methylation and cell behavioral of folic acid on ovarian cancer cell OV90. Providing theory basis for prevention and treatment of ovarian cancer.Method Ovarian cancer cells were cultured in vitro for three months and the changes of the Global genome DNA methylation, the cell growth rates, the cell cycles and the apoptosis were detected after treatment of OV90 cells with different concentration of folic acid. Ovarian cancer cells in high folic acid group were treated with 40 mg/L folic acid, cells with routine culture were served as control group, with the concentration of folic acid 4 mg/L and cells in the group were treated with no folic acid. Respectively, the methylation of genomic DNA was observed by MethyFlash Methylated DNA Quantification Kit(colorimetric), the cell grough rates were detected by CCK-8; Flow cytometry was employed to observe the changes of cell cycles and the apoptosis. Results The methylation of Global genomic DNA of OV90 increased with the concentration of folic acid; The cell grouth rates decreased obviously; Compared with control group, the cell percentage of G2/M phase in folic acid group decreased(P<0.05), the cell percentage of S phase increased (P<0.05), and there was no significant change in G0/G1 phase (P>0.05)and the cell apoptosis showed an increasing tendency. Conclusions Folic acid may increase the methylation of the Global genome DNA of ovarian cancer cell OV90 and inhibit the cell growth.