人类疱疹病毒7U41细胞内表达的探讨

Expression kineties of HHV-7 U41 gene and the localization of the U41 protein in infected cells

目的 探讨人类疱疹病毒 7(humanherpesvirus 7,HHV 7)U4 1的表达时相及细胞内定位。方法 免疫荧光检测U4 1蛋白的细胞内定位 ,RT PCR检测U4 1RNA的表达时相。结果 免疫荧光检测显示 ,无论在HHV 7感染细胞或HHV 7U4 1转染细胞 ,U4 1蛋白都是仅在其胞核中弥漫性的分布。在分别加入CHX(cycloheximide)和PFA(phosphomo formicacid)作为蛋白合成和核酸合成抑制剂感染细胞中 ,U4 1的表达时相检测结果显示U4 1蛋白在HHV 7感染细胞中是一早期表达蛋白。结论 HHV 7U4 1在病毒复制的早期表达 ,并积累于细胞核中 。

Objective To identify the expression kinetics of ORF (open reading frame) U41 of human herpesvirus 7 (HHV 7) and the localization of the U41 protein in infected cells.Methods Immunofluorescent detection was used to show the localization of the U41 protein in cells. The expression kinetics of the U41 gene in the presence of cycloheximide (CHX) and phosphomoformic acid(PFA), which are inhibitors of protein synthesis and of viral DNA synthesis respectively, was detected with RT PCR. Results The U41 protein was expressed as an early protein in the HHV 7 infected cells and localized only in the nuclei of infected cells. Conclusion These findings suggested that the function of U41 was associated with the regulation of viral DNA synthesis and replication of HHV 7.