新疆地区回族、汉族人群HBV基因型的分布与特点

The distribution and characteristics of HBV genotypes between Hui and Han population in XinJiang

目的了解新疆地区回族、汉族慢性乙型肝炎患者HBV基因型分布特点,完善新疆少数民族地区HBV流行病学资料。方法对来自乌鲁木齐和伊犁地区的回族与汉族乙型肝炎患者共194例,收集血清样本,应用ELISA法检测乙肝五项,测序方法进行HBV基因分型,荧光定量PCR法检测HBV基因载量。结果 94例回族患者中各基因型比例分别为:B型7例占7.44%,C型66例占70.21%,D型21例占22.34%。100例汉族患者中各基因型比例分别为:B型23例占23%,C型57例占57%,D型12例占12%,B/C混合型8例占8%。汉族和回族中均以C型占优势,但4种基因型在汉族和回族中的总体分布不同:C型和D型在回族的分布频率大于汉族(P=0.004和P=0.002),而B型和混合型在汉族中的分布频率大于回族(P=0.003和P=0.007)。HBV基因载量测定检测结果显示,回、汉两民族间D基因型在HBV-DNA拷贝数上有显著差异(P<0.05),其他型别均无统计学意义。结论HBV的4种基因型在汉族和回族中总体分布不同,两民族C型都占有绝对优势,但基因类型构成又各具特点,体现了新疆地区HBV基因型的地域分布和族群分布特点。

Objective To study the distribution characteristics of HBV genotypes in Hui,Han patients with chronic hepatitis B virus(HBV) and epidemiological data of HBV in Xinjiang. Methods The five serological markers were detected by ELISA,HBV genotyping was performed by genome sequencing method,HBV load were detected by fluorogenic quantitative PCR.Results Type B in 7 cases(7.44%)、type C in 66 cases(70.21%) and type D in 21 cases(22.34%) were genotyped in 94 Hui cases.Type B in 23 cases(23%), type C in 57 cases(57%), type D in 12 cases(12%) and 8 cases of BC type(8%) were genotyped in 100Han cases. The results showed that HBV genotype in two people were mostly of type C, type D ratio in Hui population was significantly higher than that in Han population. HBV gene load tests showed that there were statistically significant difference among genotype D in Hui patients. Conclusion In Xinjiang the main HBV genotype were C and D type in Hui patients while B and C type were main genotyoe in Han patients. Genotype C has an absolute advantage in both Hui and Han patients. But composition of genotypein two population had its own characteristics. It showed the ethnic and regional distribution characteristic of HBV genotype in Xinjiang minority area.