期刊文献+

Th17/Treg平衡与葡萄胎严重程度的关系 被引量:2

Correlation between Th17/Treg balance and the grades of hydatidiform mole
下载PDF
导出
摘要 目的探讨Th17/Treg平衡与葡萄胎严重程度的关系。方法收集葡萄胎患者60例(轻中重各20例),同时收集20例绒癌患者作为阳性对照,并且收集同龄段的20名健康女性作为阴性对照。对葡萄胎患者继续清宫并获得蜕膜组织,并集采外周血,采用流式细胞术检测外周血及蜕膜内Th17细胞及其胞内细胞因子IL-17、IL-23,及其功能因子RORγt的含量;同样采用流式细胞术检测Treg及其胞内功能因子IL-10、TGF-β1及Foxp3的百分含量。结果葡萄胎患者仅IL-23与正常人无显著性差异,Th17、IL-17及RORγt均显著低于正常人及绒癌患者(P<0.05)。绒癌患者Treg、IL-10、TGF-β及Foxp3均显著高于葡萄胎患者及正常人(P<0.05)。三组间的Th17/Treg平衡以葡萄胎患者最低,显著低于另外两组,三组间差异两两差异均有统计学意义(P<0.05)。随着病变严重程度加重,葡萄胎患者外周血及蜕膜内Treg数量递增,而Th17及Th17/Treg递减;外周血及蜕膜Treg均与病变程度分级呈显著正相关性;而Th17及Th17/Treg均与分级呈显著负相关性,其中相关程度最大为Th17/Treg平衡与重度的关系(以上所得r值的绝对值均大于0.7,P值均小于0.05)。结论外周血及蜕膜内Th17细胞减少、Treg增多,从而导致Th17/Treg比值变小从而向Treg倾斜,是葡萄胎的一个重要免疫学现象,并且与葡萄胎的严重程度呈显著相关性。 Objective To explore the correlation between Th17/Treg balance and the grades of hydatidiform mole. Methods 60 patients with collecting hydatidiform mole(light weight each in 20 cases), 20 cases of velvet carcinoma were collected at the same time as the positive control, and collect the same age of 20 healthy women as a negative control. Continue to qing dynasty palace and decidual tissue in patients with hydatidiform mole, and in the peripheral blood, using flow cytometry test peripheral blood and decidua in Th17 cells and intracellular cytokines IL- 17, IL- 23, and its function factor ROR gamma t content; Also using flow cytometry to detect Treg and intracellular function factor IL-10, TGF-beta 1 and percentage of Foxp3.Results The patients with hydatidiform mole only IL- and there was no significant difference with normal, Th17, IL- 17 and ROR gamma t and velvet carcinoma patients were significantly lower than normal(P<0.05). Velvet carcinoma Treg, IL-10, TGFbeta and Foxp3 were significantly higher than that of hydatidiform mole patients and normal people(P<0.05). Between the three groups of Th17/Treg balance to hydatidiform mole patient minimum, significantly lower than the other two groups, two differences between the three groups were statistically significant(P<0.05). As the lesion severity is aggravating, hydatidiform mole patient peripheral blood and decidua in Treg number increasing, and the diminishing of Th17/Treg and Th17; Peripheral blood and decidua Treg classification is significant positive correlation with pathological changes; And of Th17/Treg and Th17 and classification show significant negative correlation, the related degree of Th17/Treg maximum balance relations with severe(above the absolute value of the r value is greater than 0.7, P value is less than 0.05). Conclusion The decrease Th17 cells in the peripheral blood and decidua, Treg increased, resulting in smaller ratio of Th17/Treg to Treg tilt, hydatidiform mole is an important phenomenon of immunology, and with the severity of the hydatidiform mole has significant correlation.
出处 《分子影像学杂志》 2015年第1期26-28,共3页 Journal of Molecular Imaging
基金 湛江市科技局课题(2012C3101059)
关键词 葡萄胎 Th17/Treg平衡 流式细胞术 相关性分析 hydatidiform mole the equilibrium of Th17/Treg flow cytometry correlation analysis
  • 相关文献

参考文献10

  • 1杨金英,李大金,金莉萍,杜美蓉,贺银燕,周雯慧.反复自然流产患者外周血调节性T细胞Foxp3的表达[J].现代免疫学,2008,28(2):101-103. 被引量:18
  • 2冯凤芝,向阳,单莹,万希润,杨秀玉.葡萄胎清宫前发生侵蚀性葡萄胎肺转移患者的临床分析[J].中华妇产科杂志,2007,42(12):830-833. 被引量:13
  • 3王芳,康旭,李英勇.Th1/Th2类细胞因子与葡萄胎恶变的相关性研究[J].现代妇产科进展,2007,16(12):920-922. 被引量:2
  • 4陈巧英,李大金.CD4^+CD25^+调节性T细胞的生物学特性及功能[J].中国免疫学杂志,2007,23(1):89-92. 被引量:25
  • 5Yayan Sundara,Ekaterina Jordanova,Bethy Hernowo,Supriadi Gandamihardja,Gert Fleuren.Decidual infiltration of FoxP3+ regulatory T cells, CD3+ T cells, CD56+decidual natural killer cells and Ki-67 trophoblast cells in hydatidiform molecompared to normal and ectopic pregnancies[J]. Molecular Medicine Reports . 2012 (1)
  • 6Chen Dong.Differentiation and function of pro-inflammatory Th17 cells[J]. Microbes and Infection . 2009 (5)
  • 7Zoltan Nagymanyoki,Michael J. Callahan,Mana M. Parast,Vilmos Fulop,Samuel C. Mok,Ross S. Berkowitz.Immune cell profiling in normal pregnancy, partial and complete molar pregnancy[J]. Gynecologic Oncology . 2007 (2)
  • 8S.Knoeller,E.Lim,L.Aleta,K.Hertwig,J. W.Dudenhausen,P. C.Arck.Distribution of Immunocompetent Cells in Decidua of Controlled and Uncontrolled (Choriocarcinoma/Hydatidiform mole) Trophoblast Invasion[J]. American Journal of Reproductive Immunology . 2003 (1)
  • 9Nagymanyoki Zoltan,Callahan Michael J,Parast Mana M,Fulop Vilmos,Mok Samuel C,Berkowitz Ross S.Immune cell profiling in intraplacental and postmolar choriocarcinomas. The Journal of reproductive medicine . 2008
  • 10Scriba TJ,Kalsdorf B,Abrahams DA,et al.Distinct,specific IL-17 - and IL-22-producing CD4-+T cell subsets contribute to the human anti-mycobacterial immune response. J Immunol . 2008

二级参考文献50

  • 1[1]Tafuri A,Alferink J,Moller P,et al.T cell awareness of paternal alloantigens during pregnancy[J].Science,1995,270,630-633.
  • 2[2]Stephen LA,Mottet C,Mason D,et al.Human CD4(+)CD25(+)thymocytes and peripheral T cells have immune suppressive activity in virto[J].Eur J Immunol,2001,31:1247-1254.
  • 3[3]Aluvihare VR,Kallikourdis M,Betz AG.Tolerance,suprression and the fetal allograft[J].Mol Med,2005,83:88-96.
  • 4[4]Hori S,Nomura T,Sakaguchi S.Control of regulatory T cell development by the transcription factor Foxp3[J].Science,2003,299:1057-1061.
  • 5[5]Wang XP,Ma ZW,Hong Y,et al The skewed TCR-BV repertoire displayed at the maternal-fetal interface of women with unexplained pregnancy lossEJ].Am J Reproduc Immunol,2005,54:1-12.
  • 6[6]Sakaguchi S,Sakaguchi N,Asano M,et al.Immanologic slef-tolerance maintained by activated T cells expressing IL-2 receptor alpha chains(CD25)[J].J Immunol,1995,155(3):1151-1164.
  • 7[7]Allan SE,Passerini L,Bacchetta R,et al.The role of 2 FOXP3 isoforms in the generation of human CD4+Treg[J].J Clin Invest,2005,115(11):3276-3284.
  • 8[8]Lin W,Truong N,Grossman WJ,et al.Allergic dysregulation and hyperimmunoglobuli-nemia E in Foxp3 mutant mice[J].J Allergy Clin Immunol,2005,116(5):1106-1115.
  • 9[9]Kim JM,Rudensky A.The role of the transcription factor Foxp3 protein in the development of regulatory T cells[J].Microbes Infect,2004,6(8):745-751.
  • 10[10]Moseman EA,Liang X,Dawson AJ,et al.Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+regulatory T cells[J].J Immunol,2004,173(7):4433-4442.

共引文献54

同被引文献14

引证文献2

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部