Th17/Treg平衡与葡萄胎严重程度的关系

Correlation between Th17/Treg balance and the grades of hydatidiform mole

目的探讨Th17/Treg平衡与葡萄胎严重程度的关系。方法收集葡萄胎患者60例(轻中重各20例),同时收集20例绒癌患者作为阳性对照,并且收集同龄段的20名健康女性作为阴性对照。对葡萄胎患者继续清宫并获得蜕膜组织,并集采外周血,采用流式细胞术检测外周血及蜕膜内Th17细胞及其胞内细胞因子IL-17、IL-23,及其功能因子RORγt的含量;同样采用流式细胞术检测Treg及其胞内功能因子IL-10、TGF-β1及Foxp3的百分含量。结果葡萄胎患者仅IL-23与正常人无显著性差异,Th17、IL-17及RORγt均显著低于正常人及绒癌患者(P<0.05)。绒癌患者Treg、IL-10、TGF-β及Foxp3均显著高于葡萄胎患者及正常人(P<0.05)。三组间的Th17/Treg平衡以葡萄胎患者最低,显著低于另外两组,三组间差异两两差异均有统计学意义(P<0.05)。随着病变严重程度加重,葡萄胎患者外周血及蜕膜内Treg数量递增,而Th17及Th17/Treg递减;外周血及蜕膜Treg均与病变程度分级呈显著正相关性;而Th17及Th17/Treg均与分级呈显著负相关性,其中相关程度最大为Th17/Treg平衡与重度的关系(以上所得r值的绝对值均大于0.7,P值均小于0.05)。结论外周血及蜕膜内Th17细胞减少、Treg增多,从而导致Th17/Treg比值变小从而向Treg倾斜,是葡萄胎的一个重要免疫学现象,并且与葡萄胎的严重程度呈显著相关性。

Objective To explore the correlation between Th17/Treg balance and the grades of hydatidiform mole. Methods 60 patients with collecting hydatidiform mole(light weight each in 20 cases), 20 cases of velvet carcinoma were collected at the same time as the positive control, and collect the same age of 20 healthy women as a negative control. Continue to qing dynasty palace and decidual tissue in patients with hydatidiform mole, and in the peripheral blood, using flow cytometry test peripheral blood and decidua in Th17 cells and intracellular cytokines IL- 17, IL- 23, and its function factor ROR gamma t content; Also using flow cytometry to detect Treg and intracellular function factor IL-10, TGF-beta 1 and percentage of Foxp3.Results The patients with hydatidiform mole only IL- and there was no significant difference with normal, Th17, IL- 17 and ROR gamma t and velvet carcinoma patients were significantly lower than normal(P<0.05). Velvet carcinoma Treg, IL-10, TGFbeta and Foxp3 were significantly higher than that of hydatidiform mole patients and normal people(P<0.05). Between the three groups of Th17/Treg balance to hydatidiform mole patient minimum, significantly lower than the other two groups, two differences between the three groups were statistically significant(P<0.05). As the lesion severity is aggravating, hydatidiform mole patient peripheral blood and decidua in Treg number increasing, and the diminishing of Th17/Treg and Th17; Peripheral blood and decidua Treg classification is significant positive correlation with pathological changes; And of Th17/Treg and Th17 and classification show significant negative correlation, the related degree of Th17/Treg maximum balance relations with severe(above the absolute value of the r value is greater than 0.7, P value is less than 0.05). Conclusion The decrease Th17 cells in the peripheral blood and decidua, Treg increased, resulting in smaller ratio of Th17/Treg to Treg tilt, hydatidiform mole is an important phenomenon of immunology, and with the severity of the hydatidiform mole has significant correlation.