ACE基因I/D多态性与动脉血栓性脑梗死的关联性研究

Association between ACE gene I/D polymorphism and atherothrombotic infarction

目的研究血管紧张素转换酶(ACE)基因I/D多态位点与动脉血栓性脑梗死的关联性及相关机制。方法选取动脉血栓性脑梗死患者112例和年龄、性别匹配的对照组180例,采用PCR-AFLP技术对ACE基因I/D位点进行分型。结果单因素分析提示病例组收缩压水平(138.1±19.7 mm Hg)显著高于对照组(126.6±20.6 mm Hg)(t=4.716,P<0.001);病例组舒张压水平(88.9±10.1 mm Hg)显著高于对照组(79.9±10.0 mm Hg)(t=7.450,P<0.001)。病例组中ACE基因I/D位点D等位基因频率和DD基因型频率分布均显著高于对照组(等位基因:χ2=4.953,P=0.026;基因型:χ2=6.303,P=0.043)。病例组和对照组中ACE基因I/D位点基因型DD携带者收缩压及舒张压水平显著高于基因型ID及II携带者。结论 ACE基因I/D位点等位基因D可能是汉族人群动脉血栓性脑梗死遗传易感基因。等位基因D可能通过升高个体血压的机制导致动脉血栓性脑梗死发生。

Objective To investigate the relationship between angiotensin converting enzyme(ACE) gene I/D polymorphism and atherothrombotic infarction.Methods 112 patients with atherothrombotic infarction and 180 age,sex-matched healthy subjects were recruited.Genotypes were detected by PCR-AFLP.Results Univariate analysis revealed that systolic blood pressure(SBP) levels for patients(138.1 ± 19.7mm Hg) were significantly higher than those for healthy controls(126.6 ± 20.6mm Hg)(t=4.716,P<0.001),and diastolic blood pressure(DBP) levels for patients(88.9 ± 10.1 mm Hg) were significantly higher than those for healthy controls(79.9 ± 10.0 mm Hg)(t=7.450,P<0.001).Allele D frequency and distribution of frequency for genotype DD of ACE gene I/D polymorphism for patients were significantly higher than those for healthy controls(allele:x2=4.953,P=0.026;genotype:x2=6.303,P=0.043).In both groups,the SBP and DBP levels for genotype DD were significantly higher than those for genotype ID and II.Conclusion Allele D of ACE gene I/D polymorphism plays a role in elevating blood pressure and may be a major risk factor for atherothrombotic infarction.