摘要
本研究以p BI121为植物表达载体,在已构建的无融合生殖基因表达载体p BI121-Mh SERK1和p BI121-Mhd SERK1的基础上,利用根癌农杆菌介导法,将苹果属无融合生殖相关基因SERK1转入受体植株烟草中。研究结果表明:烟草叶片浸染后,经共培养和选择培养形成再生芽,分别获得7株p BI121-Mh SERK1和6株p BI121-Mhd SERK1转烟草抗性植株,PCR检测证明已成功获得Mh SERK1和Mhd SERK1烟草转化株系。本研究可为下一步无融合生殖相关基因功能验证研究奠定基础。
In this study, based on the constructed expression vectors of p BI121-Mh SERK1 and p BI121-Mhd SERK1,we transferred an apomixis related-gene in Malus, SERK1, to tobacco plants with p BI121 as expression vectors by Agrobacterium tumefaciens-mediated transformation. The results showed that the transgenic plants of 7individuals with p BI121-Mh SERK1 and 6 individuals with p BI121-Mhd SERK1 were obtained after co-culture and selective culture the tobacco leaves, and PCR detection demonstrated that Mh SERK1 and Mhd SERK1 were transferred into tobacco successfully. The study lay a foundation of apomixis gene proving genes function.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第6期1283-1288,共6页
Molecular Plant Breeding
基金
国家自然科学基金(30971975)资助
