花生AhFAD2的基因型与O/L值的相关性分析

Correlation between Genotype of AhFAD2 and Oleic/Linoleic Acid Value in the Different Peanut Varieties

花生(Arachis hypogaea L.)高油酸性状受2对同源非等位隐性基因ol1和ol2调控,分别编码Ah FAD2A和Ah FAD2B。本研究利用红外无损检测技术分析不同花生品种的油酸、亚油酸及粗脂肪含量,并设计等位基因特异引物,对不同油亚比值的12个花生品种进行AS-PCR检测分析。结果表明:对12个花生基因型均能准确分析出Ah FAD2基因型,其中1514、606、9102、614和1474的基因型为OL1OL1OL2OL2,其相应的O/L值位于0.971~1.759;花17、1513、1476和1586的基因型是ol1ol1/OL2OL2,其相应的O/L值位于2.252~3.679;1504、1505和1515的基因型为ol1ol1ol2ol2,其相应的O/L值位于8.204~12.79。综合12个花生品系籽粒O/L值和Ah FAD2基因型的相关性结果表明,基因型的改变直接导致O/L值的变化,但与含油量无特定相关性。

Peanut oil acid trait is controlled by the two recessive genes ol1 and il2(encoding Ah FAD2 A and Ah FAD2 B respectively). Twelve peanut cultivars with different oil and O/L ratio were selected to detect the genotype of FAD2A/FAD2 B gene to improve the peanut quality breeding of peanut breeding effectively. The results showed that the Ah FAD2 genotype of 12 lines could be accurately analyzed. The genotypes of 1514, 606,9102, 614 and 1474 are OL1OL1OL2OL2 and the corresponding O/L value is between 0.971~1.759. Hua17, 1513,1476 and 1586 are ol1ol1/OL2OL2 and the corresponding O/L value is between 2.252~3.679, 1504, 1505 and 1515 are ol1ol1ol2ol2and the corresponding O/L value is between 8.204~12.79. Ah FAD2 genotypes of the 12 peanut lines,the content of oil and the O/L value integrated showed us the difference of genotype leading to the variation of O/L directly, and the O/L value had no specific correlation with the content of oil.