山东莲藕(Nelumbo nucifera Gaertn.)种质资源的RAPD鉴定和遗传多样性分析

RAPD Identification and Genetic Diversity Analysis of Lotus(Nelumbo nucifera Gaertn.) Germplasms in Shandong Province

山东莲藕(Nelumbo nucifera Gaertn.)栽培历史悠久,在中国北方具有重要影响。但是,山东莲藕种质资源研究一直没有开展。为收集和保存莲藕种质资源,本研究从山东省8个种植面积较大的地区采集了76份莲藕材料,并利用RAPD分子标记对这些材料进行了遗传相似性分析,共鉴定出38份种质资源。遗传多样性指数分析显示,38份种质资源的Nei's多样性指数(H)平均值为0.26,Shannon信息指数(I)平均值为0.41。研究数据表明,山东莲藕种质资源相对丰富,但要建立高质量的种质资源圃,仍需要加强种质资源的引进。

Shandong province plays an important role in the north of China with a long history of lotus(Nelumbo nucifera Gaertn.), but the study on lotus germplasm resources in Shandong province has not been conducted yet. For collection and conservation of lotus germplasm, we conducted molecular marker genotyping with RAPD maker for76 lotus materials gathered from 8 districts where lotus grown in a large area in Shandong province. As a result,totally 38 lotus germplasms were identified out through the analysis of genetic similarity. Genetic diversity detection found that the average of Nei's gene diversity index is 0.26 and Shannon's information index is 0.41 for these 38 lotus germplasms. These data demonstrate that the lotus germplasm resources in Shandong province are relatively rich,while more germplasms need to be introduced from other districts to establish a high-quality lotus germplasm garden.