34份甘蔗栽培原种抗褐锈病性鉴定及Bru1基因的分子检测

Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 34 Sugarcane Cultivated Original Species

明确甘蔗栽培原种对黑顶柄锈菌的抗性水平,了解Bru1基因在甘蔗栽培原种资源中的分布状况,可以帮助筛选甘蔗原种抗源种质,提高抗褐锈病育种效率。本研究于2013年对中国国家甘蔗种质资源圃保存的34份甘蔗栽培原种进行苗期抗褐锈病鉴定和抗褐锈病基因Bru1的分子检测。苗期抗性鉴定结果表明,34份供试材料中,1级高抗至3级中抗的有26份,占76.5%。其中13份材料表现1级高抗,占38.2%,6份材料表现2级抗病,占17.6%,7份材料表现3级中抗,占20.6%。分子检测结果显示:34份供试材料中25份抗病材料含有抗褐锈病基因Bru1,出现频率为73.5%;其余1份抗病材料和8份感病材料不含抗褐锈病基因Bru1。本研究结果显示了中国国家甘蔗种质资源圃保存的甘蔗栽培原种资源中褐锈病抗性主要由Bru1控制,这一发现为利用Bru1防治由黑顶柄锈菌引起的甘蔗褐锈病提供了重要信息和参考依据。

The objectives of this study were to ascertain the level of resistance of a selection of sugarcane ancestral species to Puccinia melanocephala,and determine the distribution of the Bru1 gene in sugarcane ancestral species,and screen rust-resistant ancestral species to improve the efficiency of brown rust resistance breeding.Brown rust resistance was assessed at the seedling stage in 34 sugarcane ancestral species from the collection of the National Nursery of Sugarcane Germplasm Resources(NNSGR,Kaiyuan,China) in 2013,and 26 ancestral species(76.5%) were highly resistant(Grade 1) or moderately resistant(Grade 3). Of these,13(38.2%) were highly resistant(Grade 1),six(17.6%) were resistant(Grade 2) and seven(20.6%) were moderately resistant(Grade 3).Molecular detection indicated that Bru1 was present in 25 out of the 26 resistant varieties,and absent from the other one resistant and the eight susceptible ancestral species. These results concluded that brown rust resistance in sugarcane ancestral species which preserved in NNSGR is mainly controlled by Bru1. This finding will provide the important information and reference for utilizing Bru1 to control brown rust disease in sugarcane caused by P.melanocephala.