摘要
β-胡萝卜素羟化酶基因在植物类胡萝卜素的合成代谢过程中起着关键的作用。烟草中的类胡萝卜素是烟叶香气物质的主要前体物之一。本研究克隆了烟草4个β-胡萝卜素羟化酶基因。烟草β-胡萝卜素羟化酶基因与其它作物的BCH基因具有相似的结构,由7个外显子和6个内含子组成。其编码的蛋白包含BCH蛋白的保守结构域,即脂肪酸羟化酶超家族保守域。进化分析表明,烟草Nt BCH蛋白与番茄、枸杞、辣椒等茄科作物的BCH蛋白遗传距离较近。组织特异性表达分析表明,Nt BCH1和Nt BCH2基因主要在叶和花中表达,Nt BCH3和Nt BCH4主要在根和花中表达。从本研究结果推断,可以通过调控Nt BCH1和Nt BCH2基因的表达控制烟叶中类胡萝卜素的含量。
β-carotene hydroxylase gene(BCH) plays a key role in the process of carotenoid synthesis and metabolism in plant. The carotenoids is one of the major aroma precursor of tobacco aroma substances. This study cloned 4 tobacco β-carotene hydroxylase genes. The BCH genes of tobacco are composed of 7 exons and 6introns,have the similar structure with that of other plants. The BCH proteins contain conserved domain of fatty acid hydroxylase superfamily. Phylogenetic analysis showed that the genetic distance of Nt BCH is closed to BCH protein of tomato,Chinese wolfberry and peppers. Tissue specific expression analysis showed that Nt BCH1 and Nt BCH2 are mainly expressed in leaves and flowers,Nt BCH3 and Nt BCH4 are mainly expressed in root and flower. This study showed that tobacco carotenoid content could be control by regulating the expression of Nt BCH1 and Nt BCH2.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第8期1831-1837,共7页
Molecular Plant Breeding
基金
云南省烟草科技计划项目(2015YN01)
云南中烟工业有限责任公司科技项目(品种与品牌)共同资助
