分子标记辅助选择Pi25基因选育抗稻瘟病三系不育系

Breeding Blast-resistant Male Sterile Line of Three Line Hybrid Rice by Pi25 Gene Marker Assisted Selection

稻瘟病是水稻最具毁灭性的病害之一,选育抗稻瘟病三系不育系,是选育抗稻瘟病品种的有效途径之一。本研究以携带抗稻瘟病基因Pi25的材料BL47为抗性供体亲本、福稻B为受体亲本,利用分子标记辅助选择技术和常规育种方法,从F2代起利用分子标记Si13070C检测Pi25基因,从F3代起结合稻瘟病重发病区苗瘟鉴定,从BC1代起对不育系植株进行花粉育性鉴定,筛选出5份具有中抗以上水平的候选不育系,其中不育系CP4A具有良好的不育性和开花习性,所配组合表现出良好的农艺性状。结果表明,利用分子标记Si13070C检测Pi25基因并结合田间苗瘟鉴定在选育抗性育种材料上是有效的。

The rice blast was known as one of the most crushing diseases in rice production. One of effective ways to develop blast-resistant hybrid rice combination should be through breeding blast-resistant sterile line. In this study, five rice candidate sterile lines that have resistance or moderate resistance to rice blast were selected through the approaches of marker assisted selection and conventional breeding methods by using BL-47 as the donor parent that carries rice blast resistant gene Pi25 and Fudao B as the receptor parent. From the beginning of F2, molecular marker Si13070 C was used to detect gene Pi25; while from the beginning of F3, we began to identify seedling blast in rice blast endemic area, and from the beginning of BC1 we set about identifying pollen fertility of sterile line plants. Stigma exsertion rate of these candidate sterile lines was investigated. The results indicated that CP4 A should be highly stable in sterility and own good flowering characteristics. And the hybrids derived from CP4 A displayed elite agronomic characters. In conclusion, it would be an effective breeding process to develop blast-resistant materials by using molecular marker Si13070 C to detect gene Pi25 and combined with identifying seedling blast in rice blast endemic area.