摘要
DNA甲基化是一种重要的遗传修饰现象,植物中DNA甲基化主要是通过RNA介导的甲基化路径(RNA direct DNA methylation,Rd DM)而建立。其中ARGONAUTE 4(AGO4)和ARGONAUTE 6(AGO6)基因是该路径中重要的功能基因,它们能够结合24 nt的si RNA,目前烟草(Nicotiana tabacum L)中尚无这2个基因的报道。本文根据拟南芥(Arabidopsis thaliana)At AGO4及At AGO6的蛋白序列,通过同源比对烟草基因组数据库鉴定出烟草7个Nt AGO4及2个Nt AGO6候选基因。根据烟草基因组测序和转录组测序数据,预测了烟草Nt AGO4及Nt AGO6基因结构,结果表明候选基因具有相似的基因结构,均具有PIWI保守结构域。采用MEGA 6.0软件构建进化树,发现栽培烟草具有Nt AGO4及Nt AGO6候选基因。目前关于烟草基因信息的研究比较少,本研究丰富了对烟草AGO4及AGO6基因的认识。
DNA methylation is an important phenomenon of genetic modification and is mainly developed through the pathway of RNA direct DNA methylation(Rd DM). ARGONAUTE 4(AGO4) and ARGONAUTE 6(AGO6) genes play important roles in the Rd DM pathway, which can bind to si RNA of 24 nt. So far, there has been no report about AGO4 and AGO6 in tobacco(Nicotiana tabacum L). In the present study, seven putative Nt AGO4 genes and two Nt AGO6 genes were identified from its genome according to the homologous sequences in Arabidopsis thaliana.Prediction of gene structures showed that they share similar gene structures and conserved PIWI structure. The phylogenetic tree constructed by MEGA6.0 revealed that two genes, Nt AGO4 and Nt AGO6 were identified in tobacco. The present research will provide us some insights on AGO4 and AGO6 in tobacco, potential functions and phylogenetic relationship were further analyzed, which can provide us more information about them.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第9期2065-2068,共4页
Molecular Plant Breeding
基金
云南省烟草公司项目(2015YN02
2013YN06
2015YN08)资助
