烤烟易烤性连锁标记的适用性验证

Validation on Applicability of Markers Linked to Easy Curing Potential of Flue-cured Cured Tobacco

烤烟的易烤性影响了烘烤过程中的变黄过程,直接影响烤后烟叶的外观质量。本研究对5个与烤烟易烤性紧密连锁的分子标记进行了有效性检测。结果表明,在云烟85和大白筋599组合的F2群体中利用亲本带型进行分离群体分组,除标记PT53143外,其余4个标记其组间易烤性差异均达到显著以上水平;在自然群体中,以易烤品种云烟85的等位基因型为优异基因型,根据供试品种含有优异基因型位点的数量多寡可将24份品种分为两组,且两组之间易烤性差异达到了显著水平,进一步的遗传多样性分析揭示了易烤性较好一组的供试位点明显趋向保守,存在典型的选择牵连效应,推测供试位点附近极有可能存在与易烤性调控有关的基因。本研究不但验证了供试标记的可用性,同时也提出了一种利用供试标记进行烤烟易烤性分子鉴定和筛选的策略,为今后烤烟品种易烤性改良和新品种选育提供了一定的技术基础。

Exterior quality of flue-cured tobac co was directly subject to easy curing potential that affects the yellowing process of curing.Five markers linked to easy curing potential were tested in efficiency.The results showed that the individuals in F2 population by crossing Yunyan85 and Dabaijin599 were grouped according to the banding patterns of the two parents.easy curing potential was showed in significant level or above between two groups in four markers except for the marker PT53143.In natural population where the allele of easy curing cultivar Yun85 was defined as excellent genotype,24 materials were grouped into two according to the number of excellent allele that the tested cultivars have,and difference between the two groups was in significant level.Furthermore,the genetic diversity analysis revealed obviously that the tested locus of the better group leaned into conservative,which was a typical symbol of hitchhiking effects.It was speculated that genes related to regulating easy curing potential exists probably near these locus.In the research,the availability of the tested markers have been verified and strategies of using tested markers to make identification and selection have been suggested to supply a technique foundation for promotion of easy curing potential and breeding.