摘要
为高效筛选多小麦抗叶锈聚合品种中间材料中含有的目的基因,加速多抗品种的培育,本研究对以生产上高产优质小麦品种、含有多个高抗叶锈病基因的抗病小麦材料为亲本杂交获得的杂交优势F2代材料进行目的基因的选择,利用Lr10、Lr24、Lr34、Lr37和Lr38的分子标记对目的基因进行分子辅助鉴定。结果从根据目标性状选择的54份材料中快速选择出含有聚合目的基因Lr10、Lr34或Lr24、Lr37、Lr38或含Lr24、Lr38的聚合中间材料47份,鉴定出表现抗叶锈病,但不含有目的基因的聚合材料7份。研究减少了假阳性的选择,缩短育种中的大量筛选与鉴定的过程,提高了目的基因的选择的准确度,并为加速育种进程奠定了基础。
In order to screen the aimed pyramiding wheat leaf rust resistance genes Lr10, Lr24, Lr34, Lr37 and Lr38 high efficiently, and improve the resistance breeding process, the closely linked molecular markers corresponding to Lr10, Lr24, Lr34, Lr37 and Lr38 were used to identify resistance genes in 54 F2 plants derived from the highly yielding varieties and the lines with highly effective wheat leaf rust resistance genes. The result showed that the molecular markers were effective in selecting the aimed resistance genes in the combination of Lr10 and Lr34 or Lr24, Lr37 and Lr38 or Lr24 and Lr38, and 47 plants were selected. Seven wheat plants showed resistance were found only contain single resistance gene or no known aimed resistance gene. This result indicated that the closely linked molecular markers of the resistance genes were powerful tools to improve efficiency in pyramiding resistance breeding program.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第11期2421-2428,共8页
Molecular Plant Breeding
基金
国家重点基础研究发展计划(2013CB127700)
河北省现代农业产业技术体系--小麦产业创新团队建设项目和河北省高等学校科学技术研究(Q2012010)共同资助
