基于ISSR揭示草莓种子不同剂量γ辐射效果及遗传多样性

Effects of γ Ray Irradiation with Different Dosages on Strawberry Seeds and their Genetic Diversity Revealed by ISSR

为研究丰香草莓种子辐射诱变后培育的植株性状遗传变异程度,本研究用3种剂量梯度的γ射线(^(60)Co)对丰香草莓的种子进行辐射诱变处理,并筛选合适的ISSR引物进行辐射效果及辐射后群体的遗传多样性分析。结果表明:(1)筛选出的4个ISSR引物可用于草莓辐照选育后的遗传变异分析;4条引物共扩增出31条条带,其中24条为多态性条带,多态性比例达79.14%。(2)3个辐照群体的遗传相似均在96%以上,高于与未辐射处理对照的平均遗传相似系数,辐照群体间的基因多样度、分化指数以及分子方差变异分析结果均表明遗传变异主要是由辐照引起的。(3)对遗传相似系数进行聚类分析表明辐照群体产生了较大的遗传变异;Shannon's信息指数及Nei's遗传多样性指数分析结果显示高剂量辐照的遗传多样性也相对高。

In order to study the degree of genetic variation of plant characters that derived from the Toyonoka strawberry seeds treated by radiation mutation, we adopted 3 gradient doses of the gamma ray(^(60)Co) to treat Toyonoka strawberry seeds irradiated by ^(60)Co, screened suitable ISSR primers to detect irradiation mutant effects and analyzed genetic variation of the irradiated groups. The results presented:(1) The selected 4 ISSR primers were used to analyze the genetic variation of irradiated strawberry; 4 ISSR primers generated 31 DNA bands, in which 24 were polymorphic bands with the ratio of 79.14%;(2) The genetic similarity of three irradiation groups was more than 96%, higher than that of the control with no irradiation treatment. The analysis results about Dst, Gst and AMOVA among irradiation groups showed that genetic variation was mainly caused by irradiation.(3) Combined with the clustering analysis of the genetic similarity coefficient, it showed that irradiation groups produced a larger genetic variation. The analysis results of Shannon's information index and Nei's genetic diversity index demonstrated that the genetic diversity that treated with high dosage irradiation was relatively high.