马尾松基因组SSR标记在松属其他树种中的通用性分析

The Transferability of Pinus massoniana SSR in other Pinus species

SSR标记是林木种内遗传多样性及种质鉴别的重要分子工具,而通用性SSR标记可有效解决近缘物种标记不足、开发成本高等问题。本研究检测并分析了基于马尾松基因组测序结果开发的22个SSR多态性标记,在湿地松、白皮松及黄山松等7个松属树种中的通用性(可扩增性及多态性)。结果显示:参试标记在湿地松、白皮松、华山松、加勒比松、油松、黄山松及细叶云南松中的有效扩增率分别为68.18%、31.82%、36.63%、63.64%、100%、100%及100%;对以上7个树种各随机抽样6个植株(实生苗)进行扩增多态性初步检测,多态性标记占有效扩增标记的比例分别为86.67%、71.43%、25.00%、78.57%、90.91%、95.45%及100%。研究结果表明该22个标记绝大部分可应用于参试树种(尤其是油松,黄山松及细叶云南松)的遗传多样性及分子标记辅助育种研究中。

Microsatellite(SSR) marker is an important molecular tool for genetic diversity analysis and germplasm identification. SSRs with transferability can effectively solve the problem that lack of molecular markers in related species for the high cost of marker development. In this study, the transferability of 22 genomic-SSR markers were tested in P. elliottii, P. bungeana, P. armandii, P. caribaea, P. tabulaeformis, P. taiwanensis and P. yunnanensis var.These 22 SSRs were initially derived from genome survey sequences of P. massoniana. There were 68.18%,31.82%, 36.63%, 63.64%, 100%, 100% and 100% SSR markers can effectively amplify in 7 species mentioned above, respectively; The percentages of polymorphic markers in corresponding species were 86.67%, 71.43%,25.00%, 78.57%, 90.91%, 95.45% and 100%, while six seedlings in each of the above seven species were randomly sampled as test samples. All these results indicated that the most of given SSR markers can be applied to the genetic diversity analysis and molecular marker-assisted breeding of tested species, especially for P.tabulaeformis, P. taiwanensis and P. yunnanensis var.